盐和水的矢量运输对呼吸道上皮细胞系至关重要。
The vectorial transport of salts and water is crucial for respiratory epithelial cell lines.
作者信息
Khoufache Khaled
机构信息
Research Center, Saint-François d'Assise Hospital, Centre Hospitalier Universitaire de Québec (CHUQ), Québec City, QC, Canada.
出版信息
Respir Res. 2015 Jun 12;16(1):70. doi: 10.1186/s12931-015-0235-1.
Abstract
Primary culture of respiratory epithelial cells is useful to study the pathophysiology of respiratory diseases. However, such primary culture has been very limited because of its high dependence on the availability of biopsies and the long time required to reach confluence. Therefore, cell lines are an alternative to primary cultures because they reach confluence faster and some can maintain their differentiation abilities. However, unlike primary cultures and native tissues just some cell lines are able to polarize, with normal channel functionality and transepithelial ionic flux.
摘要
呼吸道上皮细胞的原代培养对于研究呼吸道疾病的病理生理学很有用。然而，这种原代培养非常有限，因为它高度依赖活检样本的可获得性以及达到汇合所需的较长时间。因此，细胞系是原代培养的一种替代方法，因为它们达到汇合的速度更快，并且一些细胞系能够保持其分化能力。然而，与原代培养和天然组织不同，只有一些细胞系能够极化，并具有正常的通道功能和跨上皮离子通量。
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本文引用的文献
1
Functional architecture of the CFTR chloride channel.囊性纤维化跨膜传导调节因子（CFTR）氯离子通道的功能结构
Mol Membr Biol. 2014 Feb;31(1):1-16. doi: 10.3109/09687688.2013.868055. Epub 2013 Dec 17.
2
Generation of a human airway epithelium derived basal cell line with multipotent differentiation capacity.生成具有多能分化能力的人呼吸道上皮衍生基底细胞系。
Respir Res. 2013 Dec 3;14(1):135. doi: 10.1186/1465-9921-14-135.
3
Assessing the Disease-Liability of Mutations in CFTR.评估 CFTR 突变的疾病致病变异。
Cold Spring Harb Perspect Med. 2012 Dec 1;2(12):a009480. doi: 10.1101/cshperspect.a009480.
4
The human airway epithelial basal cell transcriptome.人类气道上皮基底细胞转录组。
PLoS One. 2011 May 4;6(5):e18378. doi: 10.1371/journal.pone.0018378.
5
Primary in vitro culture of porcine tracheal epithelial cells in an air-liquid interface as a model to study airway epithelium and Aspergillus fumigatus interactions.猪气管上皮细胞在气液界面中的原代培养作为研究气道上皮与烟曲霉相互作用的模型。
Med Mycol. 2010 Dec;48(8):1049-55. doi: 10.3109/13693786.2010.496119. Epub 2010 Jul 7.
6
Functional cystic fibrosis transmembrane conductance regulator expression in cystic fibrosis airway epithelial cells by AAV6.2-mediated segmental trans-splicing.通过AAV6.2介导的片段反式剪接在囊性纤维化气道上皮细胞中实现功能性囊性纤维化跨膜传导调节因子表达。
Hum Gene Ther. 2009 Mar;20(3):267-81. doi: 10.1089/hum.2008.173.
7
Bioelectric properties of chloride channels in human, pig, ferret, and mouse airway epithelia.人、猪、雪貂和小鼠气道上皮中氯离子通道的生物电特性。
Am J Respir Cell Mol Biol. 2007 Mar;36(3):313-23. doi: 10.1165/rcmb.2006-0286OC. Epub 2006 Sep 28.
8
Expansion of cultures of human tracheal epithelium with maintenance of differentiated structure and function.人气管上皮细胞培养的扩增及分化结构与功能的维持
Biotechniques. 2005 Aug;39(2):249-55. doi: 10.2144/05392RR02.
9
Aspergillus fumigatus causes in vitro electrophysiological and morphological modifications in human nasal epithelial cells.烟曲霉可导致人鼻上皮细胞出现体外电生理和形态学改变。
Histol Histopathol. 2002 Oct;17(4):1095-101. doi: 10.14670/HH-17.1095.
10
An improved method to obtain highly differentiated monolayers of human bronchial epithelial cells.一种获得高度分化的人支气管上皮细胞单层的改进方法。
In Vitro Cell Dev Biol Anim. 1998 Jun;34(6):478-81. doi: 10.1007/s11626-998-0081-2.
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