Chow Bryna S M, Allen Terri J
Diabetic Complications Group, Baker IDI Heart and Diabetes Research Institute, Melbourne, Victoria, Australia.
Curr Protoc Mouse Biol. 2015 Jun 1;5(2):85-94. doi: 10.1002/9780470942390.mo140192.
Diabetic nephropathy (DN) is a term used to describe kidney damage cause by diabetes. With DN as one of the leading causes of end-stage renal disease worldwide, there is a strong need for appropriate animal models to study DN pathogenesis and develop therapeutic strategies. To date, most experiments are carried out in mouse models as opposed to other species for several reasons including lower cost, ease of handling, and easy manipulation of the mouse genome to generate transgenic and knockout animals. This unit provides detailed insights and technical knowledge in setting up one of the most widely used models of DN, the streptozotocin (STZ)-induced model. This model has been extensively exploited to study the mechanism of diabetic renal injury. The advantages and limitations of the STZ model and the availability of other genetic models of DN are also discussed.
糖尿病肾病（DN）是一个用于描述由糖尿病引起的肾脏损伤的术语。作为全球终末期肾病的主要病因之一，迫切需要合适的动物模型来研究DN的发病机制并制定治疗策略。迄今为止，由于成本较低、易于操作以及便于对小鼠基因组进行操作以生成转基因和基因敲除动物等多种原因，大多数实验是在小鼠模型中进行的，而非其他物种。本单元提供了关于建立最广泛使用的DN模型之一——链脲佐菌素（STZ）诱导模型的详细见解和技术知识。该模型已被广泛用于研究糖尿病肾损伤的机制。还讨论了STZ模型的优缺点以及其他DN遗传模型的可用性。
