膜蛋白固态核磁共振技术的发展。
The development of solid-state NMR of membrane proteins.
作者信息
Opella Stanley J
机构信息
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California, USA.
出版信息
Biomed Spectrosc Imaging. 2014;3(2):81-105. doi: 10.3233/BSI-140080.
Most biological functions are carried out in supramolecular assemblies. As a result of their slow reorientation in solution, these assemblies have been resistant to the widely employed solution NMR approaches. The development of solid-state NMR to first of all overcome the correlation time problem and then obtain informative high-resolution spectra of proteins in supramolecular assemblies, such as virus particles and membranes, is described here. High resolution solid-state NMR is deeply intertwined with the history of NMR, and the seminal paper was published in 1948. Although the general principles were understood by the end of the 1950s, it has taken more than fifty years for instrumentation and experimental methods to become equal to the technical problems presented by the biological assemblies of greatest interest. It is now possible to obtain atomic resolution structures of viral coat proteins in virus particles and membrane proteins in phospholipid bilayers by oriented sample solid-state NMR methods. The development of this aspect of the field of solid-state NMR is summarized in this review article.
大多数生物学功能是在超分子组装体中进行的。由于这些组装体在溶液中重排缓慢，它们一直难以用广泛使用的溶液核磁共振方法进行研究。本文介绍了固态核磁共振技术的发展，该技术首先克服了相关时间问题，进而获得了超分子组装体（如病毒颗粒和膜）中蛋白质的高分辨率信息谱。高分辨率固态核磁共振与核磁共振的历史紧密相连，开创性论文发表于1948年。尽管到20世纪50年代末人们已理解了其一般原理，但仪器和实验方法花了五十多年才足以解决最受关注的生物组装体所带来的技术问题。现在，通过定向样品固态核磁共振方法，可以获得病毒颗粒中病毒衣壳蛋白以及磷脂双分子层中膜蛋白的原子分辨率结构。本文综述了固态核磁共振领域这一方面的发展情况。
Zotero 插件