使用基于Luminex的多重检测法对涂抹在Whatman FTA Elute卡上的粪便样本进行检测，以检测与旅行者腹泻相关的肠道病原体。
Detection of enteropathogens associated with travelers' diarrhea using a multiplex Luminex-based assay performed on stool samples smeared on Whatman FTA Elute cards.
作者信息
Lalani Tahaniyat, Tisdale Michele D, Maguire Jason D, Wongsrichanalai Chansuda, Riddle Mark S, Tribble David R
机构信息
Infectious Disease Clinic, Naval Medical Center, Portsmouth, VA, USA; Infectious Disease Clinical Research Program, Uniformed Services University, Bethesda, MD, USA; Henry M Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
Infectious Disease Clinic, Naval Medical Center, Portsmouth, VA, USA; Infectious Disease Clinical Research Program, Uniformed Services University, Bethesda, MD, USA; Henry M Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
出版信息
Diagn Microbiol Infect Dis. 2015 Sep;83(1):18-20. doi: 10.1016/j.diagmicrobio.2015.05.011. Epub 2015 May 27.
Abstract
We evaluated the limits of detection (LoD) for an 11-plex PCR-Luminex assay performed on Whatman(™) FTA Elute cards smeared with stool containing pathogens associated with travelers' diarrhea. LoDs ranged from 10(2) to 10(5)CFU, PFU, or cysts/g for most pathogens except Cryptosporidium. Campylobacter and norovirus LoDs increased with prolonged storage of cards.
摘要
我们评估了在涂有含旅行者腹泻相关病原体粪便的Whatman(™) FTA Elute卡上进行的11重PCR-鲁米诺克斯检测的检测限（LoD）。除隐孢子虫外，大多数病原体的LoD范围为10(2)至10(5)CFU、PFU或囊肿/克。弯曲杆菌和诺如病毒的LoD随着卡片储存时间的延长而增加。
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