Kobayashi T, Iwasawa H
Biological Institute, Faculty of Science, Niigata University, Japan.
J Exp Zool. 1989 Dec;252(3):301-7. doi: 10.1002/jez.1402520313.
To clarify the mechanism of fluid secretion in the testes at the time of gonadotropin-induced spermiation, young Rana nigromaculata were used. As a morphological index of fluid secretion, luminal formation of the seminiferous tubules, and efferent ductules were observed. The following changes were seen by the administration of hCG or frog pituitary: first, the luminal formation of the seminiferous tubules was seen; next, tubular expansion became evident, and finally, luminal formation and expansion were observed in the efferent ductules. These changes were preceded by the separation of cell contact among Sertoli cells and of cell contact between Sertoli cells and the cells of efferent ductules only in the center and the swelling of Sertoli cells and cells of efferent ductules. With regard to the flow of fluid at the time of spermiation, the present results indicate the possibility that there is a difference in the ability for fluid secretion between Sertoli cells and the ductule cells.
为阐明促性腺激素诱导精子排出时睾丸中液体分泌的机制，使用了幼体黑斑蛙。作为液体分泌的形态学指标，观察了生精小管和输出小管的管腔形成。给予人绒毛膜促性腺激素（hCG）或蛙脑垂体后出现了以下变化：首先，观察到生精小管的管腔形成；接着，小管扩张变得明显，最后，在输出小管中观察到管腔形成和扩张。这些变化之前仅在中央区域支持细胞之间以及支持细胞与输出小管细胞之间的细胞接触分离，以及支持细胞和输出小管细胞肿胀。关于精子排出时的液体流动，目前的结果表明支持细胞和小管细胞之间的液体分泌能力可能存在差异。
