Bian Funing, Wu Yuan, Yu Shuanbao, Che Jie, Li Wenge, Shao Zhujun, Zhu Bingqing, Lu Jinxing
State Key Laboratory for Communicable Disease Prevention and Control, Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
State Key Laboratory for Communicable Disease Prevention and Control, Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Email:
Zhonghua Liu Xing Bing Xue Za Zhi. 2015 May;36(5):491-5.
To understand the species, genotypes and mating types of Cryptococcus neoformans and Cryptococcus gattii isolated from clinical samples in Guigang, Guangxi Zhuang Autonomous Region.
A total of 20 Cryptococcus strains were isolated from clinical samples in Guigang from 2009 to 2012. The biological identification was conducted by polymerase chain reaction (PCR) to amplify internal transcribed spacer (ITS) sequences. The serotypes and mating types of C. neoformans and C. gattii were identified by PCR with serotype-specific and mating type-specific primers. The genotype was characterized by PCR fingerprinting and URA5 gene restriction fragment length polymorphism (URA5-RFLP). Phenotype study included growth test at 37 °C, melanin production test and urease test.
Among the 20 strains, 19 (95%) were identified as C. neoformans varieties (var.) grubii (serotype A, mating type α, genotype VN I), and only 1 was identified as C. gattii (mating type α, genotype VG I). The results of virulence test showed that all the strains grew well at 37 °C and positive in both urease test and melanin production test.
C. neoformans var. grubii (serotype A, genotype VN I and mating type α) was the predominant pathogen causing cryptococcosis in Guigang, and C. gattii strain was also detected.
了解从广西壮族自治区贵港市临床样本中分离出的新型隐球菌和格特隐球菌的种类、基因型及交配型。
2009年至2012年期间，从贵港市临床样本中共分离出20株隐球菌。采用聚合酶链反应（PCR）扩增内部转录间隔区（ITS）序列进行生物学鉴定。使用血清型特异性引物和交配型特异性引物通过PCR鉴定新型隐球菌和格特隐球菌的血清型及交配型。通过PCR指纹图谱和URA5基因限制性片段长度多态性（URA5-RFLP）对基因型进行特征分析。表型研究包括37℃生长试验、黑色素产生试验和尿素酶试验。
20株菌株中，19株（95%）被鉴定为新型隐球菌格鲁比变种（血清型A，交配型α，基因型VN I），仅1株被鉴定为格特隐球菌（交配型α，基因型VG I）。毒力试验结果显示，所有菌株在37℃均生长良好，尿素酶试验和黑色素产生试验均为阳性。
新型隐球菌格鲁比变种（血清型A，基因型VN I，交配型α）是贵港市隐球菌病的主要致病病原体，同时也检测到了格特隐球菌菌株。
