[鼠疫耶尔森菌和假结核耶尔森菌鉴定方法的建立与评价]

[Establishment and evaluation of identification method for Yersinia pestis and Yersinia pseudotuberculosis].

作者信息

Shi Guoxiang, Zhang Zheng, Mei Lingling, Chen Jinhua, Mei Shenghua, Jin Dazhi, Zhang Zhikai, Wang Yumeng, Zhang Xiaohe, Luo Yun, Sun Jimin, Yu Dongzheng, Xia Lianxu

机构信息

Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.

Yiwu Prefecture Center for Disease Control and Prevention.

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 2015 May;36(5):496-500.

PMID:26080641

Abstract

OBJECTIVE

To establish a gene identification method of Yersinia pestis and Yersinia pseudotuberculosis for plague surveillance.

METHODS

According to the specific genomic sequences of Y. pestis and Y. pseudotuberculosis, i.e. "pestis Island (PeI)" and "pseudotuberculosis Island (PsI)" and the published genomic sequences of 12 strains of Y. pestis and 4 strains of Y. pseudotuberculosis, the specific identification primers of these sequences were designed.

RESULTS

A total of 52 strains of Y. pestis and 57 strains of Y. pseudotuberculosis and other intestinal bacteria strains were tested with PCR. Of the 5 pairs of Y. pestis identification primers, PeI2 and PeI11 were specific for Y. pestis. Besides Y. pestis, the primers PeI1, PeI3 and PeI12 could detect part of 57 Y. pseudotuberculosis strains. Of the 5 pairs of Y. pseudotuberculosis identification primers, PsI1 could detect all the 52 strains of Y. pestis and 57 strains of Y. pseudotuberculosis. PsI7, PsI16, PsI18 and PsI19 were specific for Y. pseudotuberculosis.

CONCLUSION

The primers PsI1, PeI 2 and PeI11, PsI7, PsI16, PsI18 and PsI19 can be used in the rapid identification of Y. pestis and Y. pseudotuberculosis, which can be also used to explore the circulation of atypical Y. pestis in quiescent plague foci.

摘要

目的

建立鼠疫监测中鼠疫耶尔森菌和假结核耶尔森菌的基因鉴定方法。

方法

根据鼠疫耶尔森菌和假结核耶尔森菌的特定基因组序列，即“鼠疫岛（PeI）”和“假结核岛（PsI）”以及已发表的12株鼠疫耶尔森菌和4株假结核耶尔森菌的基因组序列，设计这些序列的特异性鉴定引物。

结果

用PCR检测了52株鼠疫耶尔森菌、57株假结核耶尔森菌及其他肠道细菌菌株。在5对鼠疫耶尔森菌鉴定引物中，PeI2和PeI11对鼠疫耶尔森菌具有特异性。除鼠疫耶尔森菌外，引物PeI1、PeI3和PeI12能检测出57株假结核耶尔森菌中的部分菌株。在5对假结核耶尔森菌鉴定引物中，PsI1能检测出所有52株鼠疫耶尔森菌和57株假结核耶尔森菌。PsI7、PsI16、PsI18和PsI19对假结核耶尔森菌具有特异性。