Huo Qun, Zheng Zehui, Liu Jie, Li Chan, Hu Chunping
Department of Biochemistry, Guilin Medical University, Guilin 541004, China; Email:
Zhonghua Yi Xue Za Zhi. 2015 Mar 17;95(10):757-60.
To analyze the clinical value of the detection of serum desgamma carboxy prothrombin (DCP), golgi glycoprotein 73 (GP73), heat-shock protein 70 (HSP70) in primary hepatic carcinoma (PHC) diagnosis.
Enzyme-1inked immunosorbent assay and electrochemiluminescence immunoassay were used to detect the serum DCP, GP73, HSP70 and α-fetoprotein (AFP) levels in 35 PHC patients and 35 healthy controls.
AFP, DCP and GP73 levels in PHC patients were 13.780 (1.140-8 487.000)µg/L, 3 213.953 (2.510-53 994.602)pg/ml and 76.838 (24.500-232.875)ng/ml respectively, significantly higher than those in healthy controls (1.240 (0.605-5.310)µg/L, 104.610 (0.000-4 138.770)pg/ml and 30.770 (16.343-87.453)ng/ml, U value were 134.50, 258.00 and 168.00, all P < 0.01); HSP70 could not be detected in any objects. The area under the ROC curve of DCP or GP73 was 0.789, 0.863 respectively. The sensitivity and specificity to PHC diagnosis were 54.3% and 97.1% by DCP (2 939.4 pg/ml as cut-off value), 85.7% and 74.3% by GP73 (41.3 ng/ml as cut-off value), 45.7% and 100% by AFP (20 µg/L as cut-off value). In combined detection the sensitivity, specificity and accuracy were 88.6%, 74.3%, 81.4% by GP73/AFP and 91.4%, 71.4%, 81.4% by GP73/DCP/AFP.
GP73, DCP are new effective markers in the diagnosis of PHC, and the combined detection of GP73, DCP and AFP can improve the diagnosis value of PHC.
分析检测血清去γ羧基凝血酶原（DCP）、高尔基体糖蛋白73（GP73）、热休克蛋白70（HSP70）在原发性肝癌（PHC）诊断中的临床价值。
采用酶联免疫吸附试验和电化学发光免疫分析法检测35例PHC患者及35例健康对照者血清中DCP、GP73、HSP70及甲胎蛋白（AFP）水平。
PHC患者AFP、DCP及GP73水平分别为13.780（1.140 - 8487.000）μg/L、3213.953（2.510 - 53994.602）pg/ml和76.838（24.500 - 232.875）ng/ml，显著高于健康对照者（1.240（0.605 - 5.310）μg/L、104.610（0.000 - 4138.770）pg/ml和30.77（16.343 - 87.453）ng/ml，U值分别为134.50、258.00和168.00，均P < 0.01）；所有对象均未检测到HSP70。DCP或GP73的ROC曲线下面积分别为0.789、0.863。以DCP（临界值为2939.4 pg/ml）诊断PHC的灵敏度和特异度分别为54.3%和97.1%，以GP73（临界值为41.3 ng/ml）诊断的灵敏度和特异度分别为85.7%和74.3%，以AFP（临界值为20 μg/L）诊断的灵敏度和特异度分别为45.7%和100%。联合检测中，GP73/AFP联合检测的灵敏度、特异度和准确度分别为88.6%、74.3%、81.4%，GP73/DCP/AFP联合检测的灵敏度、特异度和准确度分别为91.4%、71.4%、81.4%。
GP73、DCP是PHC诊断的新型有效标志物，GP73、DCP与AFP联合检测可提高PHC的诊断价值。
