Wang Xiaozhan, Zhao Qingyan, Yu Shengbo, Wang Xule, Xiao Jinping, Dai Zixuan, Huang He, Tang Yanhong, Huang Congxin
Cardiovascular Research Institute of Wuhan University, Renmin Hospital of Wuhan University, Wuhan 430060, China.
Cardiovascular Research Institute of Wuhan University, Renmin Hospital of Wuhan University, Wuhan 430060, China; Email:
Zhonghua Yi Xue Za Zhi. 2015 Mar 24;95(11):860-4.
To explore the role of renal sympathetic denervation (RSD) on ventricular substrate remodeling in dogs with pacing-induced heart failure (HF).
A total of 19 dogs were randomized into 3 groups of sham-operated control (n = 7), right ventricular pacing induction of HF (n = 6) and RSD (n = 6). After 8-week pacing induction of HF. Hemodynamic variables were monitored at baseline and after HF. Masson's trichrome staining, enzyme-linked immunosorbent assay (ELISA) and Western blot were used to measure ventricular interstitial fibrosis, brain natriuretic peptide (BNP), angiotensin II (Ang II), aldosterone and transforming growth factor-beta (TGF-β).
All dogs in HF and HF+RSD groups showed increased left and right ventricular diastolic dimensions [left ventricle: (27.0 ± 2.4) vs (37.0 ± 2.8) mm, P < 0.01 and (30.0 ± 2.5) vs (36.0 ± 2.8) mm, P < 0.05; right ventricle: (11.0 ± 1.5) vs (14.0 ± 1.7) mm, P = 0.03 and (12.0 ± 1.1) vs (14.0 ± 1.2) mm, P < 0.05]. Compared with HF + RSD dogs, HF dogs had higher left ventricular end-diastolic pressure (LVEDP) [(25.0 ± 3.7) vs (3.3 ± 1.6) mmHg, P < 0.01] and more fibrous tissue (left ventricle:24.1% ± 4.8% vs 8.5% ± 1.9%, P < 0.01; right ventricle:17.2% ± 5.2% vs 11.8% ± 3.9%, P < 0.01). The levels of BNP, Ang II, aldosterone and TGF-β in ventricular tissue increased in HF dogs compared to sham-operated and HF+RSD dogs.
RSD could suppress ventricular substrate remodeling induced by long-term rapid ventricular pacing.
探讨肾交感神经去支配术（RSD）在起搏诱导的心力衰竭（HF）犬心室基质重塑中的作用。
将19只犬随机分为假手术对照组（n = 7）、右心室起搏诱导HF组（n = 6）和RSD组（n = 6）。在起搏诱导HF 8周后，于基线和HF后监测血流动力学变量。采用Masson三色染色、酶联免疫吸附测定（ELISA）和蛋白质印迹法检测心室间质纤维化、脑钠肽（BNP）、血管紧张素II（Ang II）、醛固酮和转化生长因子-β（TGF-β）。
HF组和HF+RSD组所有犬的左、右心室舒张末期内径均增加[左心室：（27.0±2.4）对（37.0±2.8）mm，P<0.01；（30.0±2.5）对（36.0±2.8）mm，P<0.05；右心室：（11.0±1.5）对（14.0±1.7）mm，P = 0.03；（12.0±1.1）对（14.0±1.2）mm，P<0.05]。与HF+RSD犬相比，HF犬的左心室舒张末期压力（LVEDP）更高[（25.0±3.7）对（3.3±1.6）mmHg，P<0.01]，纤维组织更多（左心室：24.1%±4.8%对8.5%±1.9%，P<0.01；右心室：17.2%±5.2%对11.8%±3.9%，P<0.01）。与假手术组和HF+RSD犬相比，HF犬心室组织中BNP、Ang II、醛固酮和TGF-β水平升高。
RSD可抑制长期快速心室起搏诱导的心室基质重塑。
