寡聚唾液酸和多聚唾液酸的质谱分析

Mass Spectrometric Analysis of Oligo- and Polysialic Acids.

作者信息

Galuska Christina E, Maass Kai, Galuska Sebastian P

机构信息

Institute of Biochemistry, Faculty of Medicine, University of Giessen, Friedrichstrasse 24, 35392, Giessen, Germany.

出版信息

Methods Mol Biol. 2015;1321:417-26. doi: 10.1007/978-1-4939-2760-9_28.

DOI:10.1007/978-1-4939-2760-9_28

PMID:26082238

Abstract

Oligo- and polysialic acids (oligo/polySia) are involved in numerous biological processes depending on the chain length, the comprised type of sialic acids, as well as the glycosidic linkages. Here, we describe the determination of the composition, the sequence, as well as the linkages between the sialic acid residues of lactonized oligo/polySia using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS)(/MS) and electrospray-ionization (ESI)-MS((n)).

摘要

寡聚唾液酸和多聚唾液酸（oligo/polySia）根据链长、所含唾液酸类型以及糖苷键参与众多生物过程。在此，我们描述了使用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）(/MS)和电喷雾电离（ESI）-MS((n))来测定内酯化寡聚/多聚唾液酸中唾液酸残基的组成、序列以及它们之间的连接方式。