用T7 RNA聚合酶合成的牛线粒体丝氨酸tRNA基因转录本的一些特性。

Some properties of bovine mitochondrial serine tRNA gene transcript synthesized with T7 RNA polymerase.

作者信息

Ueda T, Watanabe K

机构信息

Department of Life Chemistry, Graduate School at Nagatsuta, Tokyo Institute of Technology, Yokohama, Japan.

出版信息

Nucleic Acids Symp Ser. 1989(21):113-4.

PMID:2608455

Abstract

Bovine mitochondrial serine tRNA(AGY) gene transcript was synthesized in vitro with T7 RNA polymerase, and it was capable of being aminoacylated with mitochondrial serine tRNA synthetase. The melting profiles of the transcript was similar to those of native serine tRNA(AGY), suggesting that the higher-order structure of the transcript does not much differ from that of native serine tRNA(AGY). Several transcripts with base-substitution were also constructed and their aminoacylation capacity was investigated.

摘要

牛线粒体丝氨酸tRNA（AGY）基因转录本通过T7 RNA聚合酶在体外合成，并且它能够被线粒体丝氨酸tRNA合成酶氨酰化。该转录本的解链曲线与天然丝氨酸tRNA（AGY）的相似，这表明转录本的高级结构与天然丝氨酸tRNA（AGY）的高级结构没有太大差异。还构建了几种碱基替换的转录本，并研究了它们的氨酰化能力。