Kumazawa Y, Yokogawa T, Miura K, Watanabe K
Department of Industrial Chemistry, Faculty of Engineering, University of Tokyo, Japan.
Nucleic Acids Symp Ser. 1988(19):97-100.
Bovine mitochondrial tRNAPhe, tRNASer (AGY), and tRNASer (UCN) possessing unusual structures were purified using a new hybridization assay system and their properties in aminoacylation were examined. Bovine mitochondrial phenyl-alanyl- and seryl-tRNA synthetases could aminoacylate the same amino acid-specific tRNAs obtained not only from the mitochondria but also from other sources such as E. coli, Thermus thermophilus, bovine and yeast cytosols and archaebacteria, Sulfolobus acidocaldarius. On the contrary, none of both bacterial and cytosolic synthetases could aminoacylate the same amino acid specific tRNAs from the heterologous sources with some exceptions. We consider that the bovine mitochondrial aminoacyl-tRNA synthetases have considerably simple recognition mechanism toward the substrate tRNAs compared with the non-mitochondrial ones. This mechanism may be correlated with the occurrence of structural varieties of the mitochondrial tRNA species with unusual structures.
利用一种新的杂交分析系统纯化了具有异常结构的牛线粒体苯丙氨酸转运核糖核酸（tRNAPhe）、丝氨酸转运核糖核酸（AGY）和丝氨酸转运核糖核酸（UCN），并检测了它们在氨酰化反应中的特性。牛线粒体苯丙氨酰和丝氨酰转运核糖核酸合成酶能够使不仅从线粒体而且从其他来源（如大肠杆菌、嗜热栖热菌、牛和酵母胞质溶胶以及古细菌嗜酸热硫化叶菌）获得的相同氨基酸特异性转运核糖核酸发生氨酰化反应。相反，细菌和胞质溶胶合成酶中没有一种能够使来自异源的相同氨基酸特异性转运核糖核酸发生氨酰化反应，只有一些例外情况。我们认为，与非线粒体氨酰转运核糖核酸合成酶相比，牛线粒体氨酰转运核糖核酸合成酶对底物转运核糖核酸的识别机制相当简单。这种机制可能与具有异常结构的线粒体转运核糖核酸种类结构多样性的出现有关。
