Fujii S, Matsui S, Tomita K, Orita M, Tanaka T, Uesugi S
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Nucleic Acids Symp Ser. 1989(21):67-8.
The hetero duplex molecule, r(CGCA)d(AAAAAGCG):d(CGCTTTTTTGCG) which corresponds to Okazaki fragment was synthesized and its molecular structure has been analyzed by NMR study. The RNA strand of RNA-DNA hybrid region adopts A-form and DNA strand of the same region deviates from the standard B-form. The conformation of DNA-DNA duplex segment belongs to B-form. The hybrid-DNA duplex junction shows a structural discontinuities, A-B junction. The same conformational characteristic of oligo(dA): oligo(dT) tract as that of DNA oligomer which has same base sequence has been observed.
合成了与冈崎片段相对应的异源双链分子r(CGCA)d(AAAAAGCG):d(CGCTTTTTTGCG)，并通过核磁共振研究分析了其分子结构。RNA-DNA杂交区域的RNA链呈A构象，同一区域的DNA链偏离标准B构象。DNA-DNA双链片段的构象属于B构象。杂交-DNA双链连接处呈现结构不连续性，即A-B连接。已观察到寡聚(dA):寡聚(dT)片段与具有相同碱基序列的DNA寡聚物具有相同的构象特征。
