Sim L J, Joseph S A
Neuroendocrine Unit, University of Rochester, School of Medicine and Dentistry, NY 14642.
Peptides. 1989 Sep-Oct;10(5):1019-25. doi: 10.1016/0196-9781(89)90185-x.
Afferent projections to the nucleus raphe magnus (NRM) and dorsal raphe nucleus (DRN) were identified using retrograde transport of horseradish peroxidase conjugated wheat germ agglutinin (HRP-WGA). Neurons were labeled in important nociceptive regions including periaqueductal gray (PAG), arcuate nucleus, lateral hypothalamus and medial thalamic nuclei following both injections. We have immunocytochemically identified opiocortin/WGA neurons in the arcuate nucleus following NRM and DRN injections. Dual stained catecholamine/WGA perikarya were found in zona incerta, locus coeruleus, substantia nigra, nucleus tractus solitarius and adjacent A2, C2 and C3, lateral paragigantocellular reticular nucleus/C1 and lateral reticular nucleus/A1 following DRN injections and in zona incerta, substantia nigra, nucleus tractus solitarius/A2 and lateral reticular nucleus/A1 after NRM injections. These results provide further evidence for opiocortin and catecholamine modulation of analgesia.
利用结合辣根过氧化物酶的小麦胚凝集素（HRP-WGA）的逆行运输，确定了向中缝大核（NRM）和中缝背核（DRN）的传入投射。在两次注射后，重要的伤害感受区域（包括导水管周围灰质（PAG）、弓状核、下丘脑外侧和丘脑内侧核）中的神经元被标记。我们通过免疫细胞化学方法在向NRM和DRN注射后，在弓状核中鉴定出阿片皮质素/WGA神经元。在向DRN注射后，在未定带、蓝斑、黑质、孤束核以及相邻的A2、C2和C3、外侧旁巨细胞网状核/C1和外侧网状核/A1中发现了双染的儿茶酚胺/WGA核周体；在向NRM注射后，在未定带、黑质、孤束核/A2和外侧网状核/A1中发现了双染的儿茶酚胺/WGA核周体。这些结果为阿片皮质素和儿茶酚胺对镇痛的调节作用提供了进一步的证据。
