Bronnikov G E, Kulagina T P, Aripovskii A V, Kramarova L I
Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia,
Bull Exp Biol Med. 2015 Jun;159(2):266-8. doi: 10.1007/s10517-015-2938-1. Epub 2015 Jun 20.
Activities of mitochondrial electron transport chain enzymes NADH-CoQ oxidoreductase (complex I), cytochrome C-oxidase (complex IV), and citrate synthase were measured by spectrophotometry in m. quadriceps femoris homogenate from old rats receiving olive oil with the ration. Reduced activities of complexes I and IV in old animals were restored to the level of young animals after 6-week consumption of olive oil. Activity of citrate synthase did not change with age. Positive effect of olive oil on fatty-acid composition of the muscle tissue in old animals was demonstrated. The content of summary monounsaturated fatty acids, reduced with aging, and of summary polyunsaturated ones, increasing with age, were restored in old rats to the levels virtually not differing from the levels of young animals.
通过分光光度法测定了从接受橄榄油定量喂食的老年大鼠股四头肌匀浆中线粒体电子传递链酶NADH - 辅酶Q氧化还原酶（复合体I）、细胞色素C氧化酶（复合体IV）和柠檬酸合酶的活性。老年动物中复合体I和IV活性降低，在食用橄榄油6周后恢复到了年轻动物的水平。柠檬酸合酶的活性不随年龄变化。证实了橄榄油对老年动物肌肉组织脂肪酸组成具有积极作用。老年大鼠中随衰老而降低的总单不饱和脂肪酸含量以及随年龄增加的总多不饱和脂肪酸含量恢复到了与年轻动物水平几乎无差异的水平。
