Xu Enbo, Long Jie, Wu Zhengzong, Li Hongyan, Wang Fang, Xu Xueming, Jin Zhengyu, Jiao Aiquan
The State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan Univ, Wuxi, 214122, China.
Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan Univ, Wuxi, 214122, China.
J Food Sci. 2015 Jul;80(7):C1476-89. doi: 10.1111/1750-3841.12935. Epub 2015 Jun 18.
Enzymatic extrusion, instead of traditional steam cooking, to treat rice is an efficient and alternative pretreatment for Chinese rice wine fermentation. In order to determine the formation of volatiles in enzymatic extrusion-processed rice wine (EE), and to confirm its characteristic flavor compounds, headspace solid-phase micro-extraction followed by GC-MS was used. A total of 66 volatile compounds were identified in EE. During fermentation, most volatiles generated from enzymatic extruded rice had the similar trends with those from steam-cooked rice, but the differences in the concentration of volatiles indicated a changed balance of flavors release caused by enzymatic extrusion. Besides, the concentrations and sorts of volatiles in EEs fermented from different rice particle sizes, were not dramatically different. By principal component analysis, EE could be distinctly separated from other traditional Chinese rice wines according to its characteristic volatiles, namely, 2-heptanol, 1-octen-3-ol, ethyl 4-hydroxybenzoate, methylpentyl 2-propenoate, γ-hexalactone, and 4-vinylguaiacol.
Enzymatic extrusion liquefaction has been a popular thermal treatment for cereals, and gradually being applied in fermentation and liquor-making industry all over the world. The characterization of volatile flavor compounds in Chinese rice wine processed by enzymatic extrusion liquefaction pretreatment, might be made use not only for a better understanding of this new-type rice wine, but for the further utilization of enzymatic extrusion in other wine or alcohol production as well.
采用酶法挤压而非传统的蒸煮方式处理大米,是中国米酒发酵一种高效的替代预处理方法。为了确定酶法挤压处理的米酒(EE)中挥发性成分的形成,并确认其特征风味化合物,采用顶空固相微萃取结合气相色谱 - 质谱联用技术。在EE中总共鉴定出66种挥发性化合物。在发酵过程中,酶法挤压大米产生的大多数挥发性成分与蒸煮大米产生的挥发性成分具有相似趋势,但挥发性成分浓度的差异表明酶法挤压导致风味释放平衡发生了变化。此外,不同大米颗粒大小发酵得到的EE中挥发性成分的浓度和种类没有显著差异。通过主成分分析,EE可根据其特征挥发性成分,即2 - 庚醇、1 - 辛烯 - 3 - 醇、4 - 羟基苯甲酸乙酯、甲基戊基丙烯酸酯、γ - 己内酯和4 - 乙烯基愈创木酚,与其他传统中国米酒明显区分开来。
酶法挤压液化一直是谷物常用的热处理方法,并逐渐在全球发酵和酿酒行业得到应用。对经酶法挤压液化预处理的中国米酒中挥发性风味化合物的表征,不仅有助于更好地了解这种新型米酒,也有助于酶法挤压在其他葡萄酒或酒精生产中的进一步应用。