Yang Mingjing, Wang Jintao, Li Li, Xiong Qirui, Li Xiaofei, Zhou Xuhong
Office of Science and Technology, Yunnan University of Chinese Medicine, Kunming, China.
Front Nutr. 2025 Jul 21;12:1604754. doi: 10.3389/fnut.2025.1604754. eCollection 2025.
Compound-fermented wines integrate the aromas, flavor compounds, and nutritional components from several raw materials, enriching the flavor and texture of the final product. This study aimed to explore the influence of edible flowers on the quality of pear wine by evaluating the total phenol and flavonoid contents, antioxidant capacities, and tyrosinase inhibition abilities during the mixed fermentation of pears (P) with (PR), (PD), (PC), (PL), and Lour (PO), using standard methods The findings revealed that total phenol and flavonoid contents, antioxidant capacities, and tyrosinase inhibition abilities significantly increased in compound pear-flower wine. Specifically, total polyphenol content (TPC), total flavonoid content (TFC), the DPPH(1,1-diphenyl-2-picryl-hydrazyl radical) radical scavenging activity (DRSA), Trolox equivalent antioxidant capacity (TEAC), and ferric reducing antioxidant power (FRAP) for PR were 580.69 ± 9.51 mg of gallic acid equivalents (GAE) per liter of the sample (mg GAE/L), 600.05 ± 36.6 mg of rutin per liter of the sample (mg RE/L), 0.51 ± 0.00 μmol of Trolox equivalents (TE) per milliliter (μmol TE/mL), 10.11 ± 0.06 μmol TE/mL and 6.35 ± 0.35 μmol of Fe equivalents (FE) per milliliter (μmol FE/mL), respectively. Additionally, we further analyzed the volatile and non-volatile components of P and PR at different fermentation stages. A significant difference was observed between the non-volatile and volatile metabolites, with pear rose wine (PRW) demonstrating superior characteristics compared with pear wine (PW). Phenolic acids and flavonoids were closely associated with the formation of non-volatile metabolites, while esters, hydrocarbons, alcohols, and ketones were significantly linked to volatile formation. Notably, 2(5H)-furanone, 5-ethyl-3-hydroxy-4-methyl-, emerged as a significant aroma contributor with a relative odor activity value (ROAV) of 236,348.11, giving a sweet, fruity, nutty taste. Compared with PR, decanoic acid ethyl ester increased 634.67-fold in PRW. These findings provide a foundation for further exploration into optimized fermentation protocols, mechanistic studies on flavor and bioactive compound formation, and potential commercial applications in the functional beverage industry.
复合发酵葡萄酒融合了多种原料的香气、风味化合物和营养成分,丰富了最终产品的风味和口感。本研究旨在通过使用标准方法评估梨(P)与玫瑰(PR)、牡丹(PD)、康乃馨(PC)、百合(PL)和桂花(PO)混合发酵过程中的总酚和黄酮含量、抗氧化能力以及酪氨酸酶抑制能力,探讨可食用花卉对梨酒品质的影响。研究结果表明,复合梨花花酒中的总酚和黄酮含量、抗氧化能力以及酪氨酸酶抑制能力显著提高。具体而言,PR的总多酚含量(TPC)、总黄酮含量(TFC)、DPPH(1,1-二苯基-2-苦基肼自由基)自由基清除活性(DRSA)、Trolox等效抗氧化能力(TEAC)和铁还原抗氧化能力(FRAP)分别为每升样品580.69±9.51毫克没食子酸当量(mg GAE/L)、每升样品600.05±36.6毫克芦丁(mg RE/L)、每毫升0.51±0.00微摩尔Trolox当量(TE)(μmol TE/mL)、10.11±0.06μmol TE/mL和每毫升6.35±0.35微摩尔铁当量(FE)(μmol FE/mL)。此外,我们进一步分析了P和PR在不同发酵阶段的挥发性和非挥发性成分。非挥发性和挥发性代谢物之间存在显著差异,梨玫瑰酒(PRW)比梨酒(PW)表现出更优异的特性。酚酸和黄酮类化合物与非挥发性代谢物的形成密切相关,而酯类、烃类、醇类和酮类与挥发性成分的形成显著相关。值得注意的是,2(5H)-呋喃酮,5-乙基-3-羟基-4-甲基-,作为一种重要的香气成分出现,其相对气味活性值(ROAV)为236,348.11,具有甜、果、坚果的味道。与PR相比,PRW中的癸酸乙酯增加了634.67倍。这些发现为进一步探索优化发酵方案、风味和生物活性化合物形成的机理研究以及在功能性饮料行业的潜在商业应用提供了基础。