Wang Chunqiang, Li Chunjing, Li Hongjiao, Ma Wei, Chen Shuxiong, Zhao Yun, Rao Jiahui, Zhou Xu
College of Animal Sciences, Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University, 5333 Xian Road, Changchun, Jilin 130062, P.R. of China.
J Vet Med Sci. 2015 Nov;77(11):1419-25. doi: 10.1292/jvms.14-0617. Epub 2015 Jun 22.
Inhibins, as members of the transforming growth factor beta (TGF-β) superfamily, downregulate the synthesis and secretion of follicle-stimulating hormone (FSH) in an endocrine manner. The role of inhibin/betaglycan in the ovary regulation recently gained attention. To date, no data exist on the function of inhibin α subunit and betaglycan in cystic follicles. In this study, the expressions of inhibin α subunit and betaglycan in cystic follicles were investigated using immunohistochemistry, real-time PCR and Western blot analysis. Both inhibin α subunit and betaglycan immunoreactivities were mainly localized in the granulosa cells of follicles. Expression of inhibin α subunit and betaglycan was inferior in cystic follicles compared with that in normal large follicles. However, the result of enzyme-linked immunosorbent assay showed no significant difference in the decreasing in concentration of inhibin α subunit in cystic follicular fluid compared with the control (P>0.05). In this study, we explored the effects of FSH on betaglycan expression in granulosa cells in vitro. As expected, a significant increase in the expressions of betaglycan mRNA and protein in granulosa cells was observed in response to exogenous FSH (30 ng/ml) (P<0.05) compared with the control. Consequently, this study provides evidence that the expressions of inhibin α subunit and betaglycan are inferior in cystic follicles, and this may be caused by the decrease in FSH in the presence of a cystic follicle.
抑制素作为转化生长因子β（TGF-β）超家族的成员，以内分泌方式下调促卵泡激素（FSH）的合成和分泌。抑制素/β聚糖在卵巢调节中的作用最近受到关注。迄今为止，关于抑制素α亚基和β聚糖在囊性卵泡中的功能尚无数据。在本研究中，采用免疫组织化学、实时PCR和蛋白质印迹分析研究了抑制素α亚基和β聚糖在囊性卵泡中的表达。抑制素α亚基和β聚糖的免疫反应性主要定位于卵泡的颗粒细胞中。与正常大卵泡相比，抑制素α亚基和β聚糖在囊性卵泡中的表达较低。然而，酶联免疫吸附测定结果显示，与对照组相比，囊性卵泡液中抑制素α亚基浓度的降低无显著差异（P>0.05）。在本研究中，我们在体外探索了FSH对颗粒细胞中β聚糖表达的影响。正如预期的那样，与对照组相比，外源性FSH（30 ng/ml）刺激后，颗粒细胞中β聚糖mRNA和蛋白质的表达显著增加（P<0.05）。因此，本研究提供了证据，表明抑制素α亚基和β聚糖在囊性卵泡中的表达较低，这可能是由于存在囊性卵泡时FSH减少所致。
