基于乳糖酸克拉霉素的双选择剂体系用于毛细管电泳中碱性药物对映体拆分的研究

Study on clarithromycin lactobionate based dual selector systems for the enantioseparation of basic drugs in capillary electrophoresis.

作者信息

Yu Tao, Du Yingxiang, Chen Jiaquan, Xu Guangfu, Yang Ke, Zhang Qi, Zhang Jinjing, Du Shuaijing, Feng Zijie, Zhang Yanjie

机构信息

Department of Analytical Chemistry, China Pharmaceutical University, Nanjing, P. R. China.

Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, Nanjing, P. R. China.

出版信息

J Sep Sci. 2015 Aug;38(16):2900-6. doi: 10.1002/jssc.201400677.

DOI:10.1002/jssc.201400677

PMID:26097042

Abstract

In this paper, the use of clarithromycin lactobionate, a kind of antibiotic chiral selector, in combination with four neutral cyclodextrin derivatives (glucose-β-cyclodextrin, hydroxyethyl-β-cyclodextrin, methyl-β-cyclodextrin and hydroxypropyl-β-cyclodextrin) was reported for the first time. As a result, these dual systems gave much better resolution of nefopam (the Rs increased to 3.58, 2.72, 1.49 and 1.42, respectively) compared to the single systems. The effects of buffer pH and selector concentration on the separation of nefopam were also investigated. Additionally, some other basic drugs including metoprolol, atenolol, propranolol, bisoprolol, esmolol and ritodrine were tested for the investigation and evaluation of the enantiorecognition capability of the four dual systems. As expected, the synergistic effect was observed in four systems. Different results of these dual systems were also summarized.

摘要

本文首次报道了将一种抗生素手性选择剂乳糖酸克拉霉素与四种中性环糊精衍生物（葡萄糖-β-环糊精、羟乙基-β-环糊精、甲基-β-环糊精和羟丙基-β-环糊精）联合使用。结果表明，与单一系统相比，这些二元系统对奈福泮的拆分效果要好得多（Rs分别提高到3.58、2.72、1.49和1.42）。还研究了缓冲液pH值和选择剂浓度对奈福泮分离的影响。此外，还测试了一些其他碱性药物，包括美托洛尔、阿替洛尔、普萘洛尔、比索洛尔、艾司洛尔和利托君，以研究和评估这四种二元系统的对映体识别能力。正如预期的那样，在四个系统中均观察到了协同效应。还总结了这些二元系统的不同结果。