Wang L G, Zeng H
Zhongguo Yao Li Xue Bao. 1989 May;10(3):261-5.
Effects of camphoramine chloroacetic platinum (CCP) on DNA migration and transcription, DNA-protein and DNA interstrand cross-links induced by CCP were investigated by using agarose gel electrophoresis, alkaline elution and enzymatic techniques, respectively. Chromosome break down and migration alteration of DNa modified by CCP were observed. Plasmid pAR 436 DNA transcription was also blocked when the DNA was treated with CCP. The cross-links took place 8 h after HeLa cells were exposed to CCP 10 mumol/L and higher number of cross-links were obtained after treatment with the agent 20 mumol/L. The number of cross-links was also found to be decreased when the cell lysis was digested with proteinase K. These results suggest that CCP can also induce DNA-protein cross links. Enzymatic studies indicated that CCP preferentially attacks guanine in DNA and restriction enzymes are unable to cleave G-platinated at interval of one base to the restriction sequence.
研究了氯胺酮樟脑酸铂（CCP）对DNA迁移和转录的影响，分别采用琼脂糖凝胶电泳、碱性洗脱和酶学技术研究了CCP诱导的DNA-蛋白质交联和DNA链间交联。观察到CCP修饰的DNA的染色体断裂和迁移改变。当用CCP处理DNA时，质粒pAR 436 DNA转录也被阻断。HeLa细胞暴露于10μmol/L CCP 8小时后发生交联，用20μmol/L该试剂处理后获得更多的交联。当用蛋白酶K消化细胞裂解物时，还发现交联数减少。这些结果表明CCP也能诱导DNA-蛋白质交联。酶学研究表明，CCP优先攻击DNA中的鸟嘌呤，限制性内切酶无法在距限制性序列一个碱基的间隔处切割铂化的鸟嘌呤。
