Scicli G, Nolly H, Carretero O A, Scicli A G
Hypertension Research Division, Henry Ford Hospital, Detroit, MI.
Adv Exp Med Biol. 1989;247B:217-22. doi: 10.1007/978-1-4615-9546-5_36.
A kallikrein-like kininogenase was identified in the rat adrenal gland. Most of the enzyme was present in an inactive form, since pre-incubation with trypsin markedly increased kininogenase activity from 54.8 +/- 11.8 to 230 +/- 23.0 pg bradykinin/mg protein/min. Adrenal kininogenase was inhibited 90% by phenyl methyl sulfonyl fluoride, 92% by D-Phe-Phe-Arg-chloromethylketone, 91% by aprotinin, and only 15% by soybean trypsin inhibitor. Pre-incubation with antibodies against rat urinary kallikrein resulted in 85% inhibition. The apparent molecular weight of adrenal kininogenase on gel filtration chromatography was 33 Kd. The enzyme was strongly adsorbed to immobilized rat urinary kallikrein antibodies and required drastic conditions for elution. In canine adrenal glands, we found that there was no difference in the cortical and medullary distribution of active and inactive SBTI resistant kininogenase activity. We conclude that an enzyme which closely resembles glandular kallikrein is present in adrenal glands.
在大鼠肾上腺中鉴定出一种类激肽释放酶激肽原酶。大部分酶以无活性形式存在，因为用胰蛋白酶预孵育可使激肽原酶活性从54.8±11.8显著增加至230±23.0皮克缓激肽/毫克蛋白/分钟。肾上腺激肽原酶被苯甲基磺酰氟抑制90%，被D-苯丙氨酸-苯丙氨酸-精氨酸-氯甲基酮抑制92%，被抑肽酶抑制91%，而被大豆胰蛋白酶抑制剂仅抑制15%。用抗大鼠尿激肽释放酶抗体预孵育导致85%的抑制。凝胶过滤色谱法测得肾上腺激肽原酶的表观分子量为33千道尔顿。该酶强烈吸附于固定化大鼠尿激肽释放酶抗体，需要剧烈条件才能洗脱。在犬肾上腺中，我们发现活性和无活性的抑肽酶抗性激肽原酶活性在皮质和髓质的分布没有差异。我们得出结论，肾上腺中存在一种与腺体激肽释放酶非常相似的酶。
