Stoof Susanne P, van der Klis Fiona R M, van Rooijen Debbie M, Bogaert Debby, Trzciński Krzysztof, Sanders Elisabeth A M, Berbers Guy A M
Centre for Infectious Disease Control, National Institute of Public Health and the Environment (RIVM), Bilthoven, The Netherlands; Department of Immunology and Infectious Diseases, Wilhelmina Children's Hospital, University Medical Center, Utrecht, The Netherlands.
Centre for Infectious Disease Control, National Institute of Public Health and the Environment (RIVM), Bilthoven, The Netherlands.
Vaccine. 2015 Jul 31;33(32):3933-9. doi: 10.1016/j.vaccine.2015.06.055. Epub 2015 Jun 19.
In several countries large-scale immunization of children and young adults with Meningococcal serogroup C (MenC) conjugate vaccines has induced long-standing herd protection. Salivary antibodies may play an important role in mucosal protection against meningococcal acquisition and carriage.
To investigate antibody levels in (pre)adolescents primed 9 years earlier with a single dose of MenC-polysaccharide tetanus toxoid conjugated (MenC-TT) vaccine and the response to a booster vaccination, with special focus on age-related differences and the relation between salivary and serum antibody levels.
Nine years after priming, healthy 10- (n=91), 12- (n=91) and 15-year-olds (n=86) received a MenC-TT booster vaccination. Saliva and serum samples were collected prior to and 1 month and 1 year after vaccination. MenC-polysaccharide(MenC-PS)-specific antibody levels were measured using a fluorescent-bead-based multiplex immunoassay.
Before the booster, MenC-PS-specific IgG and IgA levels in saliva and serum were low and correlated with age at priming. The booster induced a marked increase in salivary MenC-PS-specific IgG (>200-fold), but also in IgA (∼10-fold). One year after the booster, salivary IgG and IgA had remained above pre-booster levels in all age groups (∼20-fold and ∼3-fold, respectively), with persistence of highest levels in the 15-year-olds. MenC-PS-specific IgG and IgA levels in saliva strongly correlated with the levels in serum.
Parenteral MenC-TT booster vaccination induces a clear increase in salivary MenC-PS-specific IgG and IgA levels and persistence of highest levels correlates with age. The strong correlation between serum and salivary antibody levels indicate that saliva may offer an easy and reliable tool for future antibody surveillance.
在多个国家，用C群脑膜炎球菌结合疫苗对儿童和青年进行大规模免疫接种已产生了长期的群体保护作用。唾液抗体可能在黏膜保护以防止脑膜炎球菌感染和携带方面发挥重要作用。
调查9年前接种过一剂C群脑膜炎球菌-破伤风类毒素结合（MenC-TT）疫苗的（青春期前）青少年的抗体水平以及对加强免疫接种的反应，特别关注年龄相关差异以及唾液和血清抗体水平之间的关系。
在初次接种9年后，健康的10岁（n = 91）、12岁（n = 91）和15岁（n = 86）青少年接受了MenC-TT加强免疫接种。在接种前、接种后1个月和1年采集唾液和血清样本。使用基于荧光珠的多重免疫测定法测量C群脑膜炎球菌多糖（MenC-PS）特异性抗体水平。
在加强免疫之前，唾液和血清中MenC-PS特异性IgG和IgA水平较低，且与初次接种时的年龄相关。加强免疫使唾液中MenC-PS特异性IgG（>200倍）以及IgA（约10倍）显著增加。加强免疫1年后，所有年龄组的唾液IgG和IgA仍高于加强免疫前的水平（分别约为20倍和3倍），15岁组的水平持续最高。唾液中MenC-PS特异性IgG和IgA水平与血清中的水平密切相关。
肌肉注射MenC-TT加强免疫接种可使唾液中MenC-PS特异性IgG和IgA水平明显升高，且最高水平的持续存在与年龄相关。血清和唾液抗体水平之间的强相关性表明，唾液可能为未来的抗体监测提供一种简便可靠的工具。
