从毛细胞白血病脾脏中纯化两种5型耐酒石酸酸性磷酸酶及其N端序列
Purification and N-terminal sequence of two tartrate-resistant acid phosphatases type-5 from the hairy cell leukemia spleen.
作者信息
Stĕpán J J, Lau K H, Mohan S, Kraenzlin M, Baylink D J
机构信息
Department of Medicine, Loma Linda University, CA.
出版信息
Biochem Biophys Res Commun. 1989 Dec 29;165(3):1027-34. doi: 10.1016/0006-291x(89)92705-8.
Tartrate-resistant acid phosphatases types 5a and 5b were purified from human hairy cell leukemia spleen by sequential chromatography on Phenyl-Sepharose, CM-Sepharose, concanavalin A-Sepharose, FPLC Superose-12 and FPLC Mono-S. The purification over the original tissue extract was 1150- and 3300-fold, with a yield of 2.1% and 2.5%, respectively. Gel filtration indicated an Mr of about 30000 for both forms. There was a N-terminal sequence identity between the two enzymes. However, they appeared to be different as assessed by cation exchange chromatography and amino acid composition.
通过在苯基琼脂糖、CM-琼脂糖、伴刀豆球蛋白A-琼脂糖、快速蛋白质液相色谱Superose-12和快速蛋白质液相色谱Mono-S上进行连续色谱法，从人毛细胞白血病脾脏中纯化出5a型和5b型抗酒石酸酸性磷酸酶。相对于原始组织提取物，纯化倍数分别为1150倍和3300倍，产率分别为2.1%和2.5%。凝胶过滤显示两种形式的分子量约为30000。两种酶之间存在N端序列同一性。然而，通过阳离子交换色谱法和氨基酸组成评估，它们似乎有所不同。
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