Cruz V P, Oliveira C, Foresti F
Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista - UNESP, campus de Botucatu, 18618-000 Botucatu, SP, Brasil.
Mol Biol (Mosk). 2015 May-Jun;49(3):525-8. doi: 10.7868/S0026898415030039.
5S rDNA genes of the stingray Potamotrygon motoro were PCR replicated, purified, cloned and sequenced. Two distinct classes of segments of different sizes were obtained. The smallest, with 342 bp units, was classified as class I, and the largest, with 1900 bp units, was designated as class II. Alignment with the consensus sequences for both classes showed changes in a few bases in the 5S rDNA genes. TATA-like sequences were detected in the nontranscribed spacer (NTS) regions of class I and a microsatellite (GCT) 10 sequence was detected in the NTS region of class II. The results obtained can help to understand the molecular organization of ribosomal genes and the mechanism of gene dispersion.
对黄貂鱼（Potamotrygon motoro）的5S rDNA基因进行了PCR复制、纯化、克隆和测序。获得了两类不同大小的片段。最小的片段，具有342 bp的单元，被归类为I类；最大的片段，具有1900 bp的单元，被指定为II类。与两类的共有序列比对显示5S rDNA基因中有几个碱基发生了变化。在I类的非转录间隔区（NTS）检测到类似TATA的序列，在II类的NTS区域检测到微卫星（GCT）10序列。所获得的结果有助于理解核糖体基因的分子组织和基因分散机制。
