一种具有发育可塑性的成年小鼠肾细胞系可自发产生多种成年肾结构。
A developmentally plastic adult mouse kidney cell line spontaneously generates multiple adult kidney structures.
作者信息
Webb Carol F, Ratliff Michelle L, Powell Rebecca, Wirsig-Wiechmann Celeste R, Lakiza Olga, Obara Tomoko
机构信息
Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
Immunobiology and Cancer Research, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.
出版信息
Biochem Biophys Res Commun. 2015 Aug 7;463(4):1334-1340. doi: 10.1016/j.bbrc.2015.06.130. Epub 2015 Jun 23.
Despite exciting new possibilities for regenerative therapy posed by the ability to induce pluripotent stem cells, recapitulation of three-dimensional kidneys for repair or replacement has not been possible. ARID3a-deficient mouse tissues generated multipotent, developmentally plastic cells. Therefore, we assessed the adult mouse ARID3a-/- kidney cell line, KKPS5, which expresses renal progenitor surface markers as an alternative cell source for modeling kidney development. Remarkably, these cells spontaneously developed into multicellular nephron-like structures in vitro, and engrafted into immunocompromised medaka mesonephros, where they formed mouse nephron structures. These data implicate KKPS5 cells as a new model system for studying kidney development.
尽管诱导多能干细胞的能力为再生疗法带来了令人兴奋的新可能性，但三维肾脏的重建用于修复或替代尚未实现。ARID3a缺陷的小鼠组织产生了多能、具有发育可塑性的细胞。因此，我们评估了成年小鼠ARID3a-/-肾细胞系KKPS5，其表达肾祖细胞表面标志物，作为模拟肾脏发育的替代细胞来源。值得注意的是，这些细胞在体外自发发育成多细胞肾单位样结构，并植入免疫缺陷的青鳉中肾，在那里它们形成了小鼠肾单位结构。这些数据表明KKPS5细胞是研究肾脏发育的新模型系统。
Zotero 插件