Chang Cuifang, Yang Jing, Li Xiaofang, Zhao Weiming, Li Yu, Guo Pengjuan, Wang Gaiping, Xu Cunshuan
College of Life Science, Henan Normal University, Xinxiang, 453007, People's Republic of China,
Biochem Genet. 2015 Oct;53(9-10):244-59. doi: 10.1007/s10528-015-9685-x. Epub 2015 Jun 27.
Thrombopoietin (THPO) signaling pathway regulates cell activation and many other physiological activities. To study its role in liver regeneration (LR), hepatocytes were isolated from rat regenerating livers and gene expression profile was detected using the Rat Genome 230 2.0 Array. Spectral function (E t ) and information correlation coefficient (ICC) were used to analyze gene synergy based on gene expression changes. The results showed that 35 genes related to THPO signaling pathway were significantly changed during rat LR. Functional analysis with ICC showed that five genes, STAT3, PLSCR1, CTGF, PRLR, and LCP1, played a key role in hepatocyte activation. Fourteen channels of THPO signaling pathway participated in regulating hepatocyte activation during rat LR.
血小板生成素（THPO）信号通路调节细胞活化及许多其他生理活动。为研究其在肝再生（LR）中的作用，从大鼠再生肝脏中分离出肝细胞，并使用大鼠基因组230 2.0芯片检测基因表达谱。基于基因表达变化，采用谱函数（Et）和信息相关系数（ICC）分析基因协同作用。结果显示，在大鼠肝再生过程中，35个与THPO信号通路相关的基因发生了显著变化。ICC功能分析表明，信号转导和转录激活因子3（STAT3）、磷脂 scramblase 1（PLSCR1）、结缔组织生长因子（CTGF）、催乳素受体（PRLR）和淋巴细胞胞质蛋白1（LCP1）这五个基因在肝细胞活化中起关键作用。THPO信号通路的14个通道参与调节大鼠肝再生过程中的肝细胞活化。
