Zhang Xin, Deng Yuan, Chen Feng-Li, Fang Yi-Jia, Zhang Wei, Yu Liang
Department of Hematology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an 223300, Jiangsu Province, China.
Provincial Institute for Food and Drug Control, Huai'an 223300, Jiangsu Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2015 Jun;23(3):623-6. doi: 10.7534/j.issn.1009-2137.2015.03.004.
To establish human B-acute lymphoblastic leukemia NOD/SCID mouse model.
The unirradiated mice aged 4-5 weeks were injected with Nalm-6 cells via the tail vein. Successful transplantation was assessed by the general state, bone marrow smear and histopathologic examination.
After 15 days of injection with Nalm-6 cells, the NOD/SCID mice displayed the morbidily with lethargy, hind limb paralysis, hunched back and lossing weight. The infiltration of leukemia cells could be observed in bone marrow and spleen sections.
Systemic B lineage acute leukemia animal mode1 has been successfully established in the NOD/SCID mice, which is a useful tool for studying pathogenesis of leukemia and guiding clinical chemotherapy regimens.
建立人B淋巴细胞急性白血病NOD/SCID小鼠模型。
对4-5周龄未照射的小鼠经尾静脉注射Nalm-6细胞。通过一般状态、骨髓涂片及组织病理学检查评估移植是否成功。
注射Nalm-6细胞15天后，NOD/SCID小鼠出现病态，表现为嗜睡、后肢麻痹、弓背及体重减轻。在骨髓和脾脏切片中可观察到白血病细胞浸润。
已在NOD/SCID小鼠中成功建立系统性B系急性白血病动物模型，该模型是研究白血病发病机制及指导临床化疗方案的有用工具。
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