Karr T L, Weir M P, Ali Z, Kornberg T
Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
Development. 1989 Mar;105(3):605-12. doi: 10.1242/dev.105.3.605.
By the onset of gastrulation during nuclear cycle 14 of Drosophila embryogenesis, the engrailed gene is expressed in fourteen one-cell-wide stripes. Each stripe defines the anlagen of the posterior compartment of a metameric segment. We report here several observations relating to the role and disposition of the engrailed protein during the embryonic stages that precede cellularization. We demonstrate that in embryos mutant for the engrailed gene, there were characteristic morphological abnormalities as early as the 6th cleavage cycle. In addition, the engrailed protein was detected in pre-cycle-9 embryos by Western blot analysis. When localization of engrailed protein begins during cycle 14, engrailed expression was first present in broad anterior and posterior regions before the fourteen-stripe pattern appeared.
在果蝇胚胎发育的核周期14原肠胚形成开始时， engrailed基因在14条单细胞宽的条带中表达。每条条带定义了一个体节后部区域的原基。我们在此报告了一些与engrailed蛋白在细胞化之前的胚胎阶段中的作用和分布相关的观察结果。我们证明，在engrailed基因突变的胚胎中，早在第6次卵裂周期就出现了特征性的形态异常。此外，通过蛋白质免疫印迹分析在第9周期前的胚胎中检测到了engrailed蛋白。当engrailed蛋白在第14周期开始定位时，engrailed表达首先出现在宽的前部和后部区域，然后才出现14条纹模式。
