Takeo K, Suzuno R, Tanaka T, Nakamura K
First Department of Biochemistry, Yamaguchi University School of Medicine, Ube, Japan.
Electrophoresis. 1989 Dec;10(12):813-8. doi: 10.1002/elps.1150101203.
A high resolution two-dimensional affinity electrophoresis has been developed, using capillary isoelectric focusing as the first electrophoresis and slab gel affinity electrophoresis as second electrophoresis. By this method 1-2 micrograms of anti-dinitrophenyl antibodies have been separated completely into several hundred homogeneous IgG spots. They are grouped into a number of families which are composed of several IgG spots of the same affinity to the hapten but of a different pI. It is suggested that each individual family is derived from one monoclonal antibody producing cell line.
已开发出一种高分辨率二维亲和电泳方法，该方法以毛细管等电聚焦作为第一次电泳，平板凝胶亲和电泳作为第二次电泳。通过这种方法，1 - 2微克抗二硝基苯基抗体被完全分离成数百个均一的IgG斑点。它们被分成若干个家族，每个家族由几个对半抗原亲和力相同但pI不同的IgG斑点组成。有人认为每个单独的家族都源自一个单克隆抗体产生细胞系。
