Intensive Care Unit, Zhejiang Provincial People's Hospital, Hangzhou, China Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China.
Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China.
Med Mycol. 2015 Nov;53(8):810-6. doi: 10.1093/mmy/myv043. Epub 2015 Jun 30.
Dectin-2 is a C-type lectin receptor that can recognize critical structures of fungi and involve in the host immune response after pulmonary fungal infections. We aimed to investigate the association between Dectin-2 genetic polymorphisms and cryptococcosis among a series of human immunodeficiency virus (HIV)-uninfected Chinese patients. In this case control study, a total of 251 patients with cryptococcosis and 464 healthy controls were included. One tag-single nucleotide polymorphism (SNP) (rs11045418) located at 5'-flanking region of Dectin-2 gene was selected and genotyped in this study. Among 251 patients, there were 108 (43%) meningitis patients including 73 (67.7%) healthy ones, 74 (29.5%) pulmonary infected patients including 49 (66.2%) healthy ones, and 69 (27.5%) patients with both neural and pulmonary infection including 38 (55.1%) immunocompetent ones. One hundred and forty-three (74 plus 69) patients with pulmonary cryptococcosis and 177 (108 plus 69) patients with cryptococcal meningitis were compared with controls, respectively. Three samples from 143 pulmonary infected patients failed in genotyping. There was a significant difference between 86 immunocompetent pulmonary infected patients and controls in the overdominant model (C/T vs. T/T + C/C; OR, 0.59; 95%CI, 0.37-0.94; P, .026). Similar but not significant difference was found between the overall pulmonary infected patients and the controls in the overdominant model (OR, 0.77; 95%CI, 0.52-1.12; P, .17). No such difference was found between controls and patients with cryptococcal meningitis. Our study firstly showed a genetic association between Dectin-2 and pulmonary cryptococcosis.
Dectin-2 是一种 C 型凝集素受体，可识别真菌的关键结构，并在肺部真菌感染后参与宿主免疫反应。我们旨在研究 Dectin-2 基因多态性与一系列未感染人类免疫缺陷病毒 (HIV) 的中国患者 cryptococcosis 之间的关联。在这项病例对照研究中，共纳入 251 例 cryptococcosis 患者和 464 例健康对照者。选择并对 Dectin-2 基因 5'侧翼区的一个标签单核苷酸多态性 (SNP) (rs11045418) 进行基因分型。在 251 例患者中，有 108 例 (43%) 脑膜炎患者，包括 73 例 (67.7%) 健康者，74 例 (29.5%) 肺部感染患者，包括 49 例 (66.2%) 健康者，69 例 (27.5%) 同时有神经和肺部感染的患者，包括 38 例 (55.1%) 免疫功能正常者。143 例 (74 例加 69 例) 肺部 cryptococcosis 患者和 177 例 (108 例加 69 例) cryptococcal meningitis 患者分别与对照组比较。143 例肺部感染患者中有 3 例样本基因分型失败。86 例免疫功能正常的肺部感染患者与对照组在超显性模型 (C/T 与 T/T+C/C; OR, 0.59; 95%CI, 0.37-0.94; P,.026) 中差异有统计学意义。在超显性模型中，总体肺部感染患者与对照组之间也存在类似但无统计学意义的差异 (OR, 0.77; 95%CI, 0.52-1.12; P,.17)。在 cryptococcal meningitis 患者与对照组之间未发现这种差异。本研究首次显示 Dectin-2 与肺部 cryptococcosis 之间存在遗传关联。
