Tu Zhilan, Huang Dongya, Yang Jiajun, Ojha R, Xiao Yaping, Liu Rong, Du Cui, Shen Nan, An Hedi, Yu Fei, Yue Erli, Huang Zhifang
Department of Neurology, East Hospital, Tongji University School of Medicine Shanghai 200120, China.
Department of Neurology, Sixth People's Hospital of Shanghai Jiaotong University Shanghai 200240, China.
Int J Clin Exp Med. 2015 Apr 15;8(4):5446-53. eCollection 2015.
The aim of this study was to explore the effect of dyslipidemia on intima-media thickness (IMT) of Intra- and extracranial atherosclerosis by regulating the expression of heat shock protein 70 (HSP70) in rabbits. Twenty-seven male white rabbits were randomly divided into normal control group A, high fat group B and high fat + endothelial injury operation group C (each group was 9), we measured lipids and obtained tissues from different cerebral arteries including Bilateral common carotid artery (CCA), Internal carotid artery (ICA), middle cerebral artery (MCA) and vertebral artery (VA). Pathological analysis were done, western blot analysis was used to detect the expression of HSP70 in CCA and MCA. The Serum lipid levels were overall significantly increased at 12(th) week in Group B and Group C compared to normal control (P < 0.05); at 12(th) week, the IMT of CCA and MCA in group B and C were showed significant increment compared with Group A; the correlation between HDL/CHOL/LDL and IMT of different cerebral arteries are as follows: MCA > ICA > CCA > VA; between TG and IMT of different cerebral arteries: VA > ICA > MCA > CCA; the expression of HSP70 from MCA were increased compared with CCA in group B and group C (P < 0.05). Significant positive correlations were observed between hyperlipidemia and different cerebral arteries. Hyperlipidemia has more impact on IMT of intracranial cerebral arteries. The expression of HSP70 from intracranial cerebral arteries is significantly increased. The mechanisms underlied was speculated that might be involved in inhibiting the inflammatory via HSP70.
本研究旨在通过调节兔体内热休克蛋白70（HSP70）的表达，探讨血脂异常对颅内和颅外动脉粥样硬化内膜中层厚度（IMT）的影响。将27只雄性白兔随机分为正常对照组A、高脂组B和高脂+内皮损伤手术组C（每组9只），检测血脂并获取不同脑动脉组织，包括双侧颈总动脉（CCA）、颈内动脉（ICA）、大脑中动脉（MCA）和椎动脉（VA）。进行病理分析，采用蛋白质免疫印迹法检测CCA和MCA中HSP70的表达。与正常对照组相比，B组和C组在第12周时血清脂质水平总体显著升高（P<0.05）；在第12周时，B组和C组CCA和MCA的IMT与A组相比显著增加；不同脑动脉的高密度脂蛋白/胆固醇/低密度脂蛋白与IMT的相关性如下：MCA>ICA>CCA>VA；甘油三酯与不同脑动脉IMT的相关性为：VA>ICA>MCA>CCA；B组和C组中MCA的HSP70表达高于CCA（P<0.05）。高脂血症与不同脑动脉之间存在显著正相关。高脂血症对颅内脑动脉IMT的影响更大。颅内脑动脉HSP70的表达显著增加。推测其潜在机制可能与通过HSP70抑制炎症有关。
