Oszmiański Jan, Nowicka Paulina, Teleszko Mirosława, Wojdyło Aneta, Cebulak Tomasz, Oklejewicz Krzysztof
Department of Fruit and Vegetable Processing, Wroclaw University of Environmental and Life Sciences, Wroclaw 51-630, Poland.
Faculty of Biology and Agriculture, University of Rzeszów, Rzeszów 35-601, Poland.
Int J Mol Sci. 2015 Jun 26;16(7):14540-53. doi: 10.3390/ijms160714540.
Twenty three different wild blackberry fruit samples were assessed regarding their phenolic profiles and contents (by LC/MS quadrupole time-of-flight (QTOF) and antioxidant activity (ferric reducing ability of plasma (FRAP) and 2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS)) by two different extraction methods. Thirty four phenolic compounds were detected (8 anthocyanins, 15 flavonols, 3 hydroxycinnamic acids, 6 ellagic acid derivatives and 2 flavones). In samples, where pressurized liquid extraction (PLE) was used for extraction, a greater increase in yields of phenolic compounds was observed, especially in ellagic acid derivatives (max. 59%), flavonols (max. 44%) and anthocyanins (max. 29%), than after extraction by the ultrasonic technique extraction (UAE) method. The content of phenolic compounds was significantly correlated with the antioxidant activity of the analyzed samples. Principal component analysis (PCA) revealed that the PLE method was more suitable for the quantitative extraction of flavonols, while the UAE method was for hydroxycinnamic acids.
采用两种不同的提取方法，对23个不同的野生黑莓果实样品的酚类成分和含量（通过液相色谱/质谱四极杆飞行时间质谱仪（LC/MS QTOF））以及抗氧化活性（血浆铁还原能力（FRAP）和2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS））进行了评估。检测到34种酚类化合物（8种花青素、15种黄酮醇、3种羟基肉桂酸、6种鞣花酸衍生物和2种黄酮）。在使用加压液体萃取（PLE）进行萃取的样品中，与采用超声技术萃取（UAE）方法萃取后相比，酚类化合物的产量有更大幅度的增加，尤其是鞣花酸衍生物（最高59%）、黄酮醇（最高44%）和花青素（最高29%）。酚类化合物的含量与所分析样品的抗氧化活性显著相关。主成分分析（PCA）表明，PLE方法更适合黄酮醇的定量萃取，而UAE方法更适合羟基肉桂酸的萃取。
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