INRS-ETE, Université du Québec, Canada.
Crit Rev Biotechnol. 2011 Sep;31(3):227-49. doi: 10.3109/07388551.2010.513677. Epub 2010 Nov 12.
In recent years, there has been an increasing interest in diets rich in fruits and vegetables and this is mostly due to their presumed role in the prevention of various degenerative diseases, such as cancer and cardiovascular diseases. This is mainly due to the presence of bioactive compounds, such as polyphenols, carotenoids, among others. Polyphenols are one of the main classes of secondary metabolites derived from plants offering several health benefits resulting in their use as functional foods. Prior to the use of these polyphenols in specific applications, such as food, pharmaceutical, and the cosmetic industries, they need to be extracted from the natural matrices, then analyzed and characterized. The development of an efficient procedure for the extraction, proper analysis, and characterization of phenolic compounds from different sources is a challenging task due to the structural diversity of phenolic compounds, a complex matrix, and their interaction with other cellular components. In this light, this review discusses different methods of extraction, analysis, and the structural characterization of polyphenolic compounds.
近年来,富含水果和蔬菜的饮食越来越受到关注,这主要是因为它们被认为在预防各种退行性疾病方面发挥着作用,如癌症和心血管疾病。这主要是由于生物活性化合物的存在,如多酚、类胡萝卜素等。多酚是植物次生代谢物的主要类别之一,具有多种健康益处,因此被用作功能性食品。在将这些多酚应用于特定领域,如食品、制药和化妆品行业之前,需要从天然基质中提取出来,然后进行分析和鉴定。由于多酚化合物结构多样性、基质复杂以及与其他细胞成分的相互作用,开发一种从不同来源有效提取、适当分析和鉴定酚类化合物的方法是一项具有挑战性的任务。有鉴于此,本文综述了多酚化合物的不同提取、分析和结构鉴定方法。