多天线N-聚糖类别的相对定量：将PGC-LC-ESI-MS与外切糖苷酶消化相结合
Relative quantitation of multi-antennary N-glycan classes: combining PGC-LC-ESI-MS with exoglycosidase digestion.
作者信息
Abrahams J L, Packer N H, Campbell M P
机构信息
Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia.
出版信息
Analyst. 2015 Aug 21;140(16):5444-9. doi: 10.1039/c5an00691k.
PMID:26133503
Abstract
In the search for N-glycan disease biomarkers current glycoanalytical methods may not be revealing a complete picture of precious samples, and we may be missing valuable structural information that fall outside analysis windows. We report a targeted strategy combining PGC-LC-ESI-MS with exoglycosidases to improve the relative quantitation of tri and tetra-antennary glycan classes.
摘要
在寻找N-聚糖疾病生物标志物的过程中，当前的糖分析方法可能无法全面揭示珍贵样本的情况，我们可能会遗漏分析窗口之外的有价值的结构信息。我们报告了一种将PGC-LC-ESI-MS与外切糖苷酶相结合的靶向策略，以提高三触角和四触角聚糖类别的相对定量。
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