A Significant Upregulation of miR-886-5p in High Grade and Invasive Bladder Tumors.
作者信息
Khoshnevisan Atefeh, Parvin Mahmoud, Ghorbanmehr Nasim, Hatefi Nasim, Galehdari Hamid, Ziaee Seyed Amir Mohsen, Mowla Seyed Javad
机构信息
Department of Genetics, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Department of Pathology, Labbafi-Nejad Medical Centre, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
出版信息
Urol J. 2015 Jul 1;12(3):2160-4.
PURPOSE
To investigate the expression alteration of miR-886-5p in bladder tumors and evaluating its expression level as a potential biomarker in this type of cancer.
MATERIALS AND METHODS
Formalin-fixed paraffin-embedded (FFPE) samples of bladder tumors belonging to 70 patients whom had been referred to the Shahid Labbafi-Nejad medical center were obtained from the archival collection of pathology department. After RNA extraction and cDNA synthesis, expression levels of miR-886-5p were quantified by a real-time reverse transcription polymerase chain reaction (RT-PCR) approach.
RESULTS
Our data revealed a significant upregulation (~3 times) of miR-886-5p in high grade bladder tumors, compared to the low grade ones (P < .05). Moreover, its expression level could significantly discriminate noninvasive (Ta, T1) from invasive (T-2T4) tumor stages.
CONCLUSION
Our data suggests a potential role for miR-886-5p in progression of bladder cancer.
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