Monfared Hamideh, Ziaee Seyed Amir Mohsen, Hashemitabar Mahmoud, Khayatzadeh Hamid, Kheyrollahi Vahid, Tavallaei Mahmood, Mowla Seyed Javad
Molecular Genetics Department, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran.
Urol J. 2013 Sep 26;10(3):981-7.
To investigate a potential alteration in the expression levels of transforming growth factor β (TGF-β) and miR-21 in bladder cancer tissues.
Using real-time polymerase chain reaction (PCR) method, we examined a potential correlated expression of miR-21 and TGF-β variants in 30 bladder tumors and their marginal/non-tumor biopsy specimens obtained from the same patients.
Our data revealed a significant down-regulation of TGF-β variants (P = .03) along with a non-significant alteration in the expression of miR-21 in tumor vs. non-tumor samples. However, in contrast to low-grade tumors, the expression of miR-21 was upregulated in high-grade ones, and the expression level can efficiently discriminate low-grade tumors from high-grade ones (P = .03).
In accordance to the observed similarity between TGF-β variants and miR-21 gene expression alterations in bladder tumors, treating 5637 bladder cancer cell line with TGF-β recombinant protein caused a significant upregulation of miR-21. The later finding further confirmed a correlated expression of TGF-β and miR-21 in bladder tumors.
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