Peng Tao, Wang Jingtao, Lu Jingjing, Lu Hong, Teng Junfang, Jia Yanjie
Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China.
IUBMB Life. 2017 May;69(5):315-320. doi: 10.1002/iub.1385. Epub 2015 Jul 1.
Parkinson's disease (PD) is the second most common neurodegenerative disease in humans. The hormone α-melanocyte-stimulating hormone (α-MSH) has been reported to be neuroprotective in previous studies. The aim of this study is to investigate the neuroprotective effects of α-MSH against the neurotoxicity of 1-methyl-4-phenylpyridinium (MPP+). Our results indicated that treatment with α-MSH in M17 cells attenuated MPP+-induced oxidative stress, embodied by exacerbated reactive oxygen species and protein carbonyls. In addition, we found that α-MSH could improve mitochondrial function in M17 cells through increasing the level of adenosine triphosphate and mitochondrial membrane potential. Furthermore, treatment with α-MSH restored the reduction of cell viability and the induction of lactate dehydrogenase release induced by α-MSH. Importantly, Hoechst staining results indicated that α-MSH treatment significantly reduces the number of apoptotic cells after treatment with MPP+. Mechanically, we found that α-MSH prevented apoptosis signals through reducing the level of cleaved caspase-3 and attenuating cytochrome c release. All these data imply that α-MSH produces a protective effect in PD. © 2015 IUBMB Life, 69(5):315-320, 2017.
帕金森病（PD）是人类第二常见的神经退行性疾病。先前的研究报道，激素α-黑素细胞刺激素（α-MSH）具有神经保护作用。本研究旨在探讨α-MSH对1-甲基-4-苯基吡啶离子（MPP+）神经毒性的神经保护作用。我们的结果表明，在M17细胞中用α-MSH处理可减轻MPP+诱导的氧化应激，表现为活性氧和蛋白质羰基的加剧。此外，我们发现α-MSH可通过增加三磷酸腺苷水平和线粒体膜电位来改善M17细胞的线粒体功能。此外，用α-MSH处理可恢复MPP+诱导的细胞活力降低和乳酸脱氢酶释放。重要的是，Hoechst染色结果表明，α-MSH处理可显著减少MPP+处理后凋亡细胞的数量。从机制上讲，我们发现α-MSH通过降低裂解的caspase-3水平和减弱细胞色素c释放来阻止凋亡信号。所有这些数据表明α-MSH在帕金森病中具有保护作用。©2015国际生物化学与分子生物学联盟生命科学，69(5):315 - 320，2017。
