Chopra D P, Klinger M M, Sullivan J K
Department of Biochemistry, Southern Research Institute, Birmingham, Alabama 35255-5305.
J Cell Sci. 1989 May;93 ( Pt 1):133-42. doi: 10.1242/jcs.93.1.133.
Differentiating epithelial cell cultures from human tracheobronchial epithelium have been propagated in serum-free medium. The major objective of this study was to examine the trophic effects of vitamin A on cell multiplication and morphology of the tracheal cell cultures. The cellular responses were analyzed in terms of growth kinetics, morphological and ultrastructural alterations and secretion of glycoconjugates. Cell cultures in control medium exhibited characteristics of epithelial cells including microvilli on cell surfaces, desmosomes between cells, and numerous secretory vesicles in the cytoplasm. Vitamin A at 10(-6) M and 10(-7) M inhibited cell replication and enhanced the secretion of [3H]glucosamine-labeled glycoconjugates. Further, vitamin A increased the production of plasma membrane vesicles and acquisition by the cells of a highly secretory ultrastructure. This in vitro model of human epithelial cells will be important in the investigation of various aspects of growth and differentiation.
源自人气管支气管上皮的分化上皮细胞培养物已在无血清培养基中传代培养。本研究的主要目的是检测维生素A对气管细胞培养物中细胞增殖和形态的营养作用。从生长动力学、形态和超微结构改变以及糖缀合物分泌方面分析细胞反应。对照培养基中的细胞培养物表现出上皮细胞的特征，包括细胞表面的微绒毛、细胞间的桥粒以及细胞质中的大量分泌小泡。10^(-6) M和10^(-7) M的维生素A抑制细胞复制并增强[3H]葡萄糖胺标记的糖缀合物的分泌。此外，维生素A增加了质膜小泡的产生以及细胞获得高度分泌性的超微结构。这种人上皮细胞的体外模型在研究生长和分化的各个方面将具有重要意义。
