球形红细菌废水处理中生物量积累及5-氨基乙酰丙酸（ALA）产量影响因素的优化
Optimization of Influencing Factors on Biomass Accumulation and 5-Aminolevulinic Acid (ALA) Yield in Rhodobacter sphaeroides Wastewater Treatment.
作者信息
Liu Shuli, Li Xiangkun, Zhang Guangming, Zhang Jie
机构信息
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, P.R. China.
School of Environment and Resource, Renmin University of China, Beijing 100872, P.R. China.
出版信息
J Microbiol Biotechnol. 2015 Nov;25(11):1920-7. doi: 10.4014/jmb.1505.05086.
This study aimed to optimize four factors affecting biomass accumulation and 5-aminolevulinic acid (ALA) yield together with pollutants removal in Rhodobacter sphaeroides wastewater treatment. Results showed that it was feasible to produce biomass and ALA in R. sphaeroides wastewater treatment. Microaerobic, 1,000-3,000 lux, and pH 7.0 were optimal conditions for the highest ALA yield of 4.5 ± 0.5 mg/g-biomass. Under these conditions, COD removal and biomass production rate were 93.3 ± 0.9% and 31.8 ± 0.5 mg/l/h, respectively. In addition, trace elements Fe(2+), Mg(2+), Ni(2+), and Zn(2+) further improved the ALA yield, COD removal, and biomass production rate. Specifically, the highest ALA yield (12.5 ± 0.6 mg/g-biomass) was achieved with Fe(2+) addition.
本研究旨在优化影响球形红细菌废水处理中生物量积累、5-氨基乙酰丙酸（ALA）产量以及污染物去除的四个因素。结果表明，在球形红细菌废水处理过程中生产生物量和ALA是可行的。微需氧、1000 - 3000勒克斯光照以及pH值7.0是实现最高ALA产量（4.5±0.5毫克/克生物量）的最佳条件。在这些条件下，化学需氧量（COD）去除率和生物量生产率分别为93.3±0.9%和31.8±0.5毫克/升/小时。此外，微量元素Fe(2+)、Mg(2+)、Ni(2+)和Zn(2+)进一步提高了ALA产量、COD去除率和生物量生产率。具体而言，添加Fe(2+)时实现了最高的ALA产量（12.5±0.6毫克/克生物量）。
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