通过生物刺激增强球形红杆菌的生长和类胡萝卜素的生产。
Enhancement of Rhodobacter sphaeroides growth and carotenoid production through biostimulation.
机构信息
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China..
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.; School of Environment and Resource, Renmin University of China, Beijing 100872, China.
出版信息
J Environ Sci (China). 2015 Jul 1;33:21-8. doi: 10.1016/j.jes.2015.01.005. Epub 2015 Apr 14.
Bacillus thuringiensis/cereus L2 was added as a biostimulant to enhance the biomass accumulation and carotenoid yield of Rhodobacter sphaeroides using wastewater as the culturing medium. Results showed that biostimulation could significantly enhance the R. sphaeroides biomass production and carotenoid yield. The optimal biostimulant proportion was 40 μL (about 6.4×10(5) CFU). Through the use of biostimulation, chemical oxygen demand removal, R. sphaeroides biomass production, carotenoid concentration, and carotenoid yield were improved by 178%, 67%, 214%, and 70%, respectively. Theoretical analysis revealed that there were two possible reasons for such increases. One was that biostimulation enhanced the R. sphaeroides wastewater treatment efficiency. The other was that biostimulation significantly decreased the peroxidase activity in R. sphaeroides. The results showed that the highest peroxidase activity dropped by 87% and the induction ratio of the RSP_3419 gene was 3.1 with the addition of biostimulant. The enhanced carotenoid yield in R. sphaeroides could thus be explained by a decrease in peroxidase activity.
苏云金芽孢杆菌/蜡样芽孢杆菌 L2 被添加为生物刺激剂，以利用废水作为培养介质来提高球形红假单胞菌的生物量积累和类胡萝卜素产量。结果表明，生物刺激可以显著提高球形红假单胞菌的生物量生产和类胡萝卜素产量。最佳生物刺激剂比例为 40 μL（约 6.4×10(5) CFU）。通过使用生物刺激剂，化学需氧量去除率、球形红假单胞菌生物量、类胡萝卜素浓度和类胡萝卜素产量分别提高了 178%、67%、214%和 70%。理论分析揭示了这种增加的两个可能原因。一种是生物刺激增强了球形红假单胞菌的废水处理效率。另一种是生物刺激显著降低了球形红假单胞菌中的过氧化物酶活性。结果表明，添加生物刺激剂后，过氧化物酶活性最高下降了 87%，RSP_3419 基因的诱导比为 3.1。因此，可以通过降低过氧化物酶活性来解释球形红假单胞菌中类胡萝卜素产量的增加。
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