Bardsley D W, Coakley W T, Jones G, Liddell J E
School of Pure and Applied Biology, University of Wales College of Cardiff, U.K.
J Biochem Biophys Methods. 1989 Oct;19(4):339-48. doi: 10.1016/0165-022x(89)90065-1.
A technique is described in which erythrocytes suspended in 1.1 ml of 145 mM NaCl, have been fused by electrofusion. The cells in suspension were brought into close contact by setting up a 3 MHz ultrasonic standing wave in a cylindrical cell container. The aluminium foil base of the container served both to transmit ultrasound and as an electrode for electrofusion. The electric pulse was generated by a capacitor discharge system. The electric field strength required to fuse cells increased as the ionic strength of the cell suspending phase increased. Cells in physiological saline fused at an electric field strength of 7.3 kV/cm with a 50 microseconds pulse.
本文描述了一种技术，其中悬浮于1.1毫升145毫摩尔氯化钠溶液中的红细胞通过电融合法进行融合。通过在圆柱形细胞容器中建立3兆赫兹的超声驻波，使悬浮液中的细胞紧密接触。容器的铝箔底部既用于传输超声波，又作为电融合的电极。电脉冲由电容放电系统产生。随着细胞悬浮相离子强度的增加，融合细胞所需的电场强度也增加。生理盐水中的细胞在电场强度为7.3千伏/厘米、脉冲为50微秒的情况下融合。
