Grandes P, Streit P
Brain Research Institute, University of Zurich, Switzerland.
J Neurocytol. 1989 Oct;18(5):685-93. doi: 10.1007/BF01187087.
The pattern of glutamate-like immunoreactivity was investigated in the medial nucleus of the trapezoid body of adult rats. A monoclonal 'anti-glutamate' antibody was combined with postembedding immunohistochemistry involving a silver-intensified peroxidase-antiperoxidase procedure for light microscopy or an immunogold method for electron microscopy. The most conspicuous glutamate-immunoreactive elements were labelled calyces of Held. Because of the outstanding size of these terminals they may provide an attractive model for studying the details of glutamatergic neurotransmission at a synapse of the mammalian CNS.
研究了成年大鼠梯形体内侧核中谷氨酸样免疫反应性的模式。一种单克隆“抗谷氨酸”抗体与包埋后免疫组织化学相结合，其中包括用于光学显微镜的银增强过氧化物酶-抗过氧化物酶程序或用于电子显微镜的免疫金方法。最明显的谷氨酸免疫反应性元素是被标记的 Held 壶腹。由于这些终末的突出大小，它们可能为研究哺乳动物中枢神经系统突触处谷氨酸能神经传递的细节提供一个有吸引力的模型。
