Chew Muhd Hasyim, Rahman Md Mostafizur, Hussin Salasawati
Muhammad Hasyim Chew, Ph.D scholar, Laboratory Public Health Kota Kinabalu, Sabah, Ministry of Health, Malaysia.
Dr. Md. Mostafizur Rahman, Professor, Department of Medical Microbiology and Immunology, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia.
Pak J Med Sci. 2015;31(3):615-20. doi: 10.12669/pjms.313.6340.
Detection of different serotypes of dengue virus and provide information on origin, distribution and genotype of the virus.
Dengue virus serotypes identified as DEN-1 and DEN-2 were amplified and sequenced with E gene. The consensus sequences were aligned with references E gene sequences of globally available GenBank. Phylogenetic analysis was performed using Neighbor-joining and Kimura 2-parameter model to construct phylogenetic tree.
A total of 53 dengue virus isolates were positive, of which 38 (71.7%) were DENV-1 and 15 (28.3%) were DENV-2. Phylogenetic tree of DENV-1 and DENV-2 showed that the isolates were clustered in genotype I and cosmopolitan genotype, respectively considered the predominant genotypes in Southeast Asian countries. The molecular epidemiology genotype I DENV-1 and cosmopolitan genotype DENV-2 have been co-circulating in Klang Valley areas, Malaysia without shifting of genotype.
The study reveals that DENV-1 and DENV-2 have been circulating in Malaysia. The isolates are clustered in genotype 1 and cosmopolitian genotype, respectively. The study results would help in planning for prevention and control of dengue virus in Malaysia.
检测登革病毒的不同血清型，并提供有关该病毒的起源、分布和基因型的信息。
对鉴定为DEN-1和DEN-2的登革病毒血清型进行E基因扩增和测序。将获得的一致性序列与全球可获得的GenBank中的参考E基因序列进行比对。使用邻接法和Kimura双参数模型进行系统发育分析，构建系统发育树。
共有53株登革病毒分离株呈阳性，其中38株（71.7%）为DENV-1，15株（28.3%）为DENV-2。DENV-1和DENV-2的系统发育树表明，这些分离株分别聚集在基因型I和世界基因型中，这两种基因型分别被认为是东南亚国家的主要基因型。分子流行病学研究表明，基因型I的DENV-1和世界基因型的DENV-2在马来西亚巴生谷地区共同流行，且基因型未发生转变。
该研究表明DENV-1和DENV-2在马来西亚流行。分离株分别聚集在基因型1和世界基因型中。研究结果将有助于马来西亚登革病毒的预防和控制规划。
