补充硒可诱导滋养层细胞中的线粒体生物合成。
Selenium supplementation induces mitochondrial biogenesis in trophoblasts.
作者信息
Khera Alisha, Dong Lan-Feng, Holland Olivia, Vanderlelie Jessica, Pasdar Elham A, Neuzil Jiri, Perkins Anthony V
机构信息
School of Medical Science, Menzies Health Institute Queensland, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
School of Medical Science, Menzies Health Institute Queensland, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
出版信息
Placenta. 2015 Aug;36(8):863-9. doi: 10.1016/j.placenta.2015.06.010. Epub 2015 Jun 23.
INTRODUCTION
Placental oxidative stress has been implicated in pregnancy complications and previous work has shown that selenium can protect trophoblast mitochondria from oxidative stress. This report examines mitochondrial function and content in trophoblasts supplemented with selenium.
METHODS
Swan-71, JEG-3 and BeWo cells and placental tissue were incubated with sodium selenite or selenomethionine. Mitochondrial function was examined in a respirometer. Mitochondrial content was determined using RT-PCR. The levels of the mitochondrial biogenesis markers selenoprotein H, PGC-1α and NRF-1 was examined by western blotting.
RESULTS
Mitochondrial respiration was significantly enhanced post selenium supplementation in cells and tissues. Selenium supplementation increased mitochondrial content and up-regulated mitochondrial biogenesis mediators in cells.
DISCUSSION
These results emphasise the importance of selenium in mitochondrial regeneration in trophoblasts.
引言
胎盘氧化应激与妊娠并发症有关，先前的研究表明硒可以保护滋养层线粒体免受氧化应激。本报告研究了补充硒的滋养层细胞中的线粒体功能和含量。
方法
将Swan-71、JEG-3和BeWo细胞以及胎盘组织与亚硒酸钠或硒代蛋氨酸一起孵育。在呼吸计中检测线粒体功能。使用RT-PCR测定线粒体含量。通过蛋白质印迹法检测线粒体生物发生标志物硒蛋白H、PGC-1α和NRF-1的水平。
结果
补充硒后，细胞和组织中的线粒体呼吸显著增强。补充硒增加了细胞中的线粒体含量并上调了线粒体生物发生介质。
讨论
这些结果强调了硒在滋养层细胞线粒体再生中的重要性。
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