原位邻近连接分析（PLA）。

In Situ Proximity Ligation Assay (PLA).

作者信息

Bagchi Sonchita, Fredriksson Robert, Wallén-Mackenzie Åsa

机构信息

Department of Neuroscience, Uppsala biomedicinska centrum BMC, Uppsala University, Husarg. 3, 593, Uppsala, 751 24, Sweden.

出版信息

Methods Mol Biol. 2015;1318:149-59. doi: 10.1007/978-1-4939-2742-5_15.

DOI:10.1007/978-1-4939-2742-5_15

PMID:26160573

Abstract

In situ proximity ligation assay (PLA) is a method to identify physical closeness of proteins, where a signal will only be produced if the two proteins are closer than 40 nm, in tissue section or cell cultures. Modifications of the PLA method can also be used to increase specificity or sensitivity of standard immunohistochemistry protocols.

摘要

原位邻近连接分析（PLA）是一种用于识别蛋白质物理接近程度的方法，在组织切片或细胞培养物中，只有当两种蛋白质的距离小于40纳米时才会产生信号。PLA方法的改进也可用于提高标准免疫组织化学方案的特异性或灵敏度。