葡萄糖诱导的线粒体谷胱甘肽还原参与胰腺β细胞胰岛素分泌的第一阶段。
Glucose-induced glutathione reduction in mitochondria is involved in the first phase of pancreatic β-cell insulin secretion.
作者信息
Liu Xiaojing, Han Shuai, Yang Ying, Kang Jiuhong, Wu Jiarui
机构信息
Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China; Clinical and Translational Research Center of Shanghai First Maternity and Infant Health Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Science and Technology, Tongji University, Shanghai 200092, China.
Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
出版信息
Biochem Biophys Res Commun. 2015 Aug 28;464(3):730-6. doi: 10.1016/j.bbrc.2015.07.016. Epub 2015 Jul 8.
Glucose can acutely reduce mitochondrial glutathione redox state in rat islets. However, whether glucose-stimulated mitochondrial glutathione redox state relates to glucose-stimulated insulin secretion (GSIS) remains unknown. We used genetically encoded redox-sensitive GFPs to target the mitochondria to monitor glutathione redox changes during GSIS in rat pancreatic β-cells. The results showed that mitochondrial glutathione was more reduced during GSIS, whereas inhibition of this glutathione reduction impaired insulin secretion. In isolated rat pancreatic islets glutathione reduction in mitochondria and the first phase of GSIS were concurrence at the early stage of glucose-stimulation. Our results suggest that the glucose-induced glutathione reduction in mitochondria is primarily required for the first phase of GSIS.
葡萄糖可急性降低大鼠胰岛中线粒体谷胱甘肽的氧化还原状态。然而，葡萄糖刺激的线粒体谷胱甘肽氧化还原状态是否与葡萄糖刺激的胰岛素分泌（GSIS）相关仍不清楚。我们使用基因编码的对氧化还原敏感的绿色荧光蛋白靶向线粒体，以监测大鼠胰腺β细胞在GSIS过程中的谷胱甘肽氧化还原变化。结果表明，在GSIS过程中线粒体谷胱甘肽的还原程度更高，而抑制这种谷胱甘肽还原会损害胰岛素分泌。在分离的大鼠胰腺胰岛中，线粒体谷胱甘肽的还原与GSIS的第一阶段在葡萄糖刺激早期同时发生。我们的结果表明，线粒体中葡萄糖诱导的谷胱甘肽还原是GSIS第一阶段的主要必要条件。
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