栀子苷平衡氧化还原信号以介导胰腺β细胞中葡萄糖刺激的胰岛素分泌。

Geniposide Balances the Redox Signaling to Mediate Glucose-Stimulated Insulin Secretion in Pancreatic β-Cells.

作者信息

Liu Chunyan, Hao Yanan, Yin Fei, Liu Jianhui

机构信息

Chongqing Key Laboratory of Medicinal Chemistry & Molecular Pharmacology, Chongqing University of Technology, Chongqing 400054, People's Republic of China.

出版信息

Diabetes Metab Syndr Obes. 2020 Feb 25;13:509-520. doi: 10.2147/DMSO.S240794. eCollection 2020.

PURPOSE

To investigate the effect of geniposide on the biosynthesis of insulin and the expression protein disulfide isomerase (PDI) and endoplasmic reticulum oxidoreductin 1 (ERO1) in the presence of low (5 mM) and high (25 mM) glucose in pancreatic β cells.

METHODS

The content of insulin was measured by ELISA, the number of SH groups was determined with the classical chromogenic reagent, 5,5'-dithiobis-(2-nitrobenzoic) acid (DTNB; also known as Ellman's reagent), the expressions of PDI and ERO1 were analyzed by Western blot.

RESULTS

Geniposide played contrary roles on the accumulation of HO, the ratio of GSH/GSSG and the thiol-disulfide balance in the presence of low (5 mM) and high (25 mM) glucose in rat pancreatic INS-1 cells. Geniposide also regulated the protein levels of protein disulfide isomerase (PDI) and endoplasmic reticulum oxidoreductin1 (ERO1), the two key enzymes for the production of HO during the biosynthesis of insulin in INS-1 cells.

CONCLUSION

Geniposide affects glucose-stimulated insulin secretion by modulating the thiol-disulfide balance that is controlled by the redox signaling in pancreatic β cells.

目的

研究栀子苷在低（5 mM）和高（25 mM）葡萄糖条件下对胰腺β细胞中胰岛素生物合成以及蛋白二硫键异构酶（PDI）和内质网氧化还原酶1（ERO1）表达的影响。

方法

采用酶联免疫吸附测定法（ELISA）检测胰岛素含量，用经典显色剂5,5'-二硫代双（2-硝基苯甲酸）（DTNB，也称为埃尔曼试剂）测定巯基数量，通过蛋白质印迹法分析PDI和ERO1的表达。

结果

在低（5 mM）和高（25 mM）葡萄糖条件下，栀子苷对大鼠胰腺INS-1细胞中HO的积累、谷胱甘肽/氧化型谷胱甘肽（GSH/GSSG）比值及硫醇-二硫键平衡发挥相反作用。栀子苷还调节了蛋白二硫键异构酶（PDI）和内质网氧化还原酶1（ERO1）的蛋白质水平，这两种酶是INS-1细胞胰岛素生物合成过程中产生HO的关键酶。