Ben Peiling, Zhang Zhengping, Xuan Chunxia, Sun Shasha, Shen Lei, Gao Yanhong, Cao Xiang, Zhou Yi, Lan Lei, Yin Zhimin, Luo Lan
Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing, 210046, Jiangsu, People's Republic of China.
Neurochem Res. 2015 Aug;40(8):1661-70. doi: 10.1007/s11064-015-1648-4. Epub 2015 Jul 12.
L-Theanine is an amino acid derivative from green tea. The present work was aimed at the effect of L-theanine on neuron-like rat pheochromocytoma (PC12) cells stimulated with cadmium chloride. Treatment with L-theanine before cadmium exposure increased cell viability; the experiments of Annexin V/PI staining indicated that L-theanine inhibited cadmium-induced cell apoptosis. Meanwhile, L-theanine decreased ROS production and protected from cadmium-induced disruption of mitochondrial transmembrane potential. Compared with cadmium-treated cells, L-theanine could also decrease the ratio of Bax/Bcl-2, as well as the level of cleaved caspase-9, caspase-3 and poly(ADP-ribose) polymerase. Furthermore, L-theanine depresses cadmium-induced up regulation of phosphorylations of PI3K/Akt, MAPK ERK1/2, and JNK signaling. These data suggest that L-theanine pretreatment reduces severity of cadmium toxicity probably via antioxidant action. Therefore, it may be concluded that L-theanine could be exploited for prevention of cadmium-induced diseases.
L-茶氨酸是一种来自绿茶的氨基酸衍生物。本研究旨在探讨L-茶氨酸对氯化镉刺激的大鼠嗜铬细胞瘤（PC12）细胞样神经元的影响。在镉暴露前用L-茶氨酸处理可提高细胞活力；膜联蛋白V/碘化丙啶染色实验表明，L-茶氨酸可抑制镉诱导的细胞凋亡。同时，L-茶氨酸可降低活性氧的产生，并保护细胞免受镉诱导的线粒体跨膜电位破坏。与镉处理的细胞相比，L-茶氨酸还可降低Bax/Bcl-2的比值，以及裂解的半胱天冬酶-9、半胱天冬酶-3和聚（ADP-核糖）聚合酶的水平。此外，L-茶氨酸可抑制镉诱导的PI3K/Akt、MAPK ERK1/2和JNK信号通路磷酸化上调。这些数据表明，L-茶氨酸预处理可能通过抗氧化作用降低镉毒性的严重程度。因此，可以得出结论，L-茶氨酸可用于预防镉诱导的疾病。
