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p39R861 - 4,一种携带A/C1质粒RA1片段的2A/C2型质粒。

p39R861-4, A Type 2 A/C2 Plasmid Carrying a Segment from the A/C1 Plasmid RA1.

作者信息

Anantham Sashindran, Harmer Christopher J, Hall Ruth M

机构信息

School of Molecular Bioscience, The University of Sydney , Sydney, Australia .

出版信息

Microb Drug Resist. 2015 Dec;21(6):571-6. doi: 10.1089/mdr.2015.0133. Epub 2015 Jul 13.

Abstract

The largest plasmid in the strain 39R861, which is used as a plasmid size standard, was recovered by conjugation and sequenced to determine its exact size. Plasmid p39R861-4 transferred at high frequency. Although reported to be the A/C1 plasmid RA1, p39R861-4 is a 155794-bp Type 2 A/C2 plasmid, in which a 39-kb segment, derived from RA1 that includes a relative of the RA1 resistance island, replaces 26.5 kb of the Type 2 backbone. p39R861-4 includes a single copy of IS10 and two resistance islands with a CR2-sul2 region in each of them. The 84 kb of backbone between the resistance islands is inverted relative to other known A/C plasmids and this inversion has arisen through recombination between the CR2-sul2 regions that are inversely oriented. The two resistance islands present before this inversion occurred were one related to but longer than that found in RA1, and one that is a form of the ARI-B island and identical to ARI-B in the A/C2 plasmid R55. They contain genes conferring resistance to tetracycline (tetA(D)), sulfonamides (sul2), and florfenicol and chloramphenicol (floR). The tet(D) determinant is flanked by two IS26 in a transposon-like structure named Tntet(D). Both resistance islands contain remnants of the two ends of the integrative element GIsul2, consistent with the sul2 gene being mobilized by GIsul2 rather than by CR2.

摘要

用作质粒大小标准的39R861菌株中的最大质粒,通过接合回收并测序以确定其确切大小。质粒p39R861-4高频转移。尽管据报道是A/C1质粒RA1,但p39R861-4是一个155794 bp的2型A/C2质粒,其中一个源自RA1的39 kb片段(包括RA1抗性岛的一个相关物)取代了2型主干的26.5 kb。p39R861-4包含一个IS10单拷贝和两个抗性岛,每个抗性岛都有一个CR2-sul2区域。抗性岛之间的84 kb主干相对于其他已知的A/C质粒是反向的,这种反向是通过反向定向的CR2-sul2区域之间的重组产生的。在这种反向发生之前存在的两个抗性岛,一个与RA1中发现的相关但更长,另一个是ARI-B岛的一种形式,与A/C2质粒R55中的ARI-B相同。它们包含赋予对四环素(tetA(D))、磺胺类药物(sul2)以及氟苯尼考和氯霉素(floR)抗性的基因。tet(D)决定簇两侧是转座子样结构Tntet(D)中的两个IS26。两个抗性岛都包含整合元件GIsul2两端的残余部分,这与sul2基因由GIsul2而非CR2移动一致。

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