线粒体ATP合酶体：其组分的表达与结构相互作用
Mitochondrial ATP synthasome: Expression and structural interaction of its components.
作者信息
Nůsková Hana, Mráček Tomáš, Mikulová Tereza, Vrbacký Marek, Kovářová Nikola, Kovalčíková Jana, Pecina Petr, Houštěk Josef
机构信息
Department of Bioenergetics, Institute of Physiology of the Czech Academy of Sciences, Vídeňská 1083, 14220 Prague, Czech Republic; Charles University in Prague, First Faculty of Medicine, Kateřinská 32, 12108 Prague, Czech Republic.
Department of Bioenergetics, Institute of Physiology of the Czech Academy of Sciences, Vídeňská 1083, 14220 Prague, Czech Republic.
出版信息
Biochem Biophys Res Commun. 2015 Aug 28;464(3):787-93. doi: 10.1016/j.bbrc.2015.07.034. Epub 2015 Jul 10.
Mitochondrial ATP synthase, ADP/ATP translocase (ANT), and inorganic phosphate carrier (PiC) are supposed to form a supercomplex called ATP synthasome. Our protein and transcript analysis of rat tissues indicates that the expression of ANT and PiC is transcriptionally controlled in accordance with the biogenesis of ATP synthase. In contrast, the content of ANT and PiC is increased in ATP synthase deficient patients' fibroblasts, likely due to a post-transcriptional adaptive mechanism. A structural analysis of rat heart mitochondria by immunoprecipitation, blue native/SDS electrophoresis, immunodetection and MS analysis revealed the presence of ATP synthasome. However, the majority of PiC and especially ANT did not associate with ATP synthase, suggesting that most of PiC, ANT and ATP synthase exist as separate entities.
线粒体ATP合酶、ADP/ATP转位酶（ANT）和无机磷酸载体（PiC）被认为可形成一种名为ATP合酶体的超复合物。我们对大鼠组织进行的蛋白质和转录本分析表明，ANT和PiC的表达受转录调控，与ATP合酶的生物合成相一致。相比之下，ATP合酶缺陷患者的成纤维细胞中ANT和PiC的含量增加，这可能是由于转录后适应性机制所致。通过免疫沉淀、蓝色非变性/SDS电泳、免疫检测和质谱分析对大鼠心脏线粒体进行的结构分析揭示了ATP合酶体的存在。然而，大多数PiC，尤其是ANT并未与ATP合酶结合，这表明大多数PiC、ANT和ATP合酶以独立的实体形式存在。
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